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Stimulation of enzymes involved in tylosin biosynthesis by cyclic feeding profiles in fed batch cultures

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Summary

Previous reports have shown that feeding glucose and monosodium glutamate in a cyclic fashion to Streptomyces fradiae fermentations greatly stimulated the specific production rate for tylosin (qtylosin) over that which could be obtained in fermentations where the nutrients were fed at a constant rate, all other conditions being the same. In this report it was shown that the activities of three of the enzymes involved in tylosin biosynthesis, propionyl-coenzyme A carboxylase (EC 6.4.1.3), methylmalonyl-coenzyme A carboxyltransferase (EC 2.1.3.1) and macrocin 3′-0-methyl-transferase, were stimulated in the case of the cyclic fed-batch fermentation when compared with control levels i.e. the cyclic feed stimulated the activities of enzymes on the tylosin pathway as well as the qtylosin values.

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Authors and Affiliations

  1. School of Biotechnology University of New South Wales, 2033, Kensington, N.S.W., Australia

    K. Vu-Trong & P. P. Gray

Authors
  1. K. Vu-Trong
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  2. P. P. Gray
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Vu-Trong, K., Gray, P.P. Stimulation of enzymes involved in tylosin biosynthesis by cyclic feeding profiles in fed batch cultures. Biotechnol Lett 6, 435–440 (1984). https://doi.org/10.1007/BF00129305

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