Summary
The propene isolated strain Xanthobacter Py2 was able to grow on 1-pentene. The biomass yield for growth under 1-pentene limiting conditions was 0.48 (Ceq/Ceq). Upon storage at both +4°C and -20°C no loss of enzymatic epoxide degrading activity in resting cell suspensions was observed after a month. However, activity decay was pronounced during the stationary phase of growth as well as under reaction conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ginkel van CG, Bont de JAM (1986) Isolation and characterization of alkene-utilizing Xanthobacter spp. Arch Microbiol 145: 403–407.
Ginkel van CG, Welten HGJ, Bont de JAM (1986) Epoxidation of alkenes by alkene-grown Xanthobacter spp. Appl Microbiol Biotechnol 24: 334–337.
Ginkel van CG, Habets-Crützen AQH, Last van der ARM, Bont de JAM (1987) A description of microbial growth on gaseous alkenes in a chemostat culture. Biotech and Bioeng 30: 799–804.
Habets-Crützen AQH, Brink LES, Ginkel van CG, Bont de JAM, S. Tramper J (1984) Production of epoxides from gaseous alkenes by resting-cell suspensions and immobilized cells of alkene-utilizing bacteria. Appl Microbiol Biotechnol 20: 245–250.
Habets-Crützen AQH, Carlier SJN, Bont de JAM, Wistuba D, Schurig V, Hartmans S, Tramper J (1985) Stereospecific formation of 1,2-epoxypropane. 1,2-epoxybutane and 1-chloro-2,3-epoxypropane by alkene utilizing bacteria. Enzyme Microb Technol 7: 17–21.
Laane C, Boeren S, Hilhorst R, Veeger C (1987) Optimization of biocatalysis in organic media in Biocatalysis in Organic Media (Laane C, Tramper J, Lilly MD, Eds.), Elsevier Science Publishers, Amsterdam, The Netherlands, pp 65–84.
Liley PE, Reid RC, Buck E (1984) Physical and chemical data in Perry's Chemical Engineers' Handbook, 6th edition (Perry RH, Green DW, Eds.) McGraw-Hill, New York, Section 3, p59.
Lowry DH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193: 265–275.
Pirt SJ (1975) Principles of Microbe and Cell Cultivation. Blackwell Scientific Publications, Oxford, Chapter 8, pp 63–70.
Riet van't K,Tramper J (1991) Basic Bioreactor Design. Marcel Dekker, New York, USA, Chapter 3, pp51–67.
Sinclair, CG (1987) Microbiol process kinetics in Basic Biotechnology (J Bu'lock and B. Kristiansen, Eds.), Academic Press, London.
Spencer CF, Adler SB (1978) A critical review of equations for predicting saturated liquid density. J Chem Eng Data 23: 82–89.
Spencer CF, Danner RP (1972) Improved equation for prediction of saturated liquid density. J Chem Eng Data 17: 236–241.
Weijers CAGM, Haan de A, Bont de JAM (1988) Chiral resolution of 2,3-epoxyalkanes by Xanthobacter Py2. Appl Microbiol Biotechnol 27: 337–340.
Weijers CAGM, Bont de JAM (1991) Enantioselective degradation of 1,2-epoxyalkanes by Nocardia H8. Enzyme Microb Technol 13: 306–308.
Wiegant WM, Bont de JAM (1980) A new route for ethylene glycol metabolism in Mycobacterium E44. J Gen Microbiol 120: 325–331.
Yaws CL, Haur-Chung Yang, Hopper JR, Hansen KC (1990) Hydrocarbons: water solubility data. Chem Eng 97 (4): 177–182.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Van Keulen, F., Da Fonseca, M.M.R. Batch cultivation of Xanthobacter Py2 on 1-pentene. Biotechnol Lett 16, 989–994 (1994). https://doi.org/10.1007/BF00128639
Issue Date:
DOI: https://doi.org/10.1007/BF00128639