Summary
The simultaneous saccharification and fermentation (SSF) of sweet sorghum carbohydrates to ethanol by Fusarium oxysporum F3 alone or in mixed culture with Saccharomyces cerevisiae 2541 or Zymomonas mobilis CP4 in a fed-batch fermentation process was studied. While SSF was adequately carried out by the first microorganism the process achieved its maximum value by the mixed culture of the fungus and yeast. Under optimum conditions, ethanol yields and concentrations as high as 29.7 g of ethanol per 100 g of dry sorghum stalk and 7.5 % (w/v) respectively were obtained. These values together with the high yield of sorghum crop in Greece make this process promising and worthy of further investigation for the production of fuel bioethanol.
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References
Antonopoulos, A.A, and Wene, C.E. (1984). Phytopathology, 74, 1268.
Bernfeld, P. (1959). Amylases α and β. Methods Enzymol., 2, 27–29.
Bryan, W.L. (1990). Enzyme Microb. Technol., 12, 437–442.
Christakopoulos, P, Macris, B.J., and Kekos, D. (1989). Enzyme Microb. Technol. 11, 236–239.
Christakopoulos, P., Koullas, D.P., Kekos, D, Koukios, E.G., and Macris, B.J. (1991a). Enzyme Microb. Technol. 13, 272–274.
Christakopoulos, P., Macris, B.J., and Kekos, D. (1990). Appl. Microbiol. Biotechnol. 33, 18–20.
Christakopoulos, P., Koullas, D.P., Kekos, D., Koukios, E.G., and Macris, B.J. (1991b). Bioresour. Technol., 35, 297–301.
Christakopoulos, P., Li, L-W., Kekos, D., and Macris, B.J. (1993). Bioresour. Technol., 45, 98–92.
Collura, M.A., and Luyben, W.L. (1988). Ind. Eng. Chem. Res. 27,1686–1696.
Dalianis, C., Christou, M., Sooter, C., Kyritsis, S., Zafiris, C. and Samiotakis, G. (1992). In: Biomass for Energy and Industry. D.O. Hall, G. Grassi, H. Scheer, eds. pp 1220–1228, Ponte Press.
Deshpande, V., Sulbha, K., Mishra, C., and Rao, M. (1986). Enzyme Microb. Technol., 8, 149–152.
Jackman, E.A. (1987). In: Basic Biotechnology. J. Bu'Lock, B. Kristiansen, eds. pp 309–336, Academic Press Inc.
Jasberg, B.K., Montgomery, R.R., and Anderson R.A. (1983). Biotechnol. Bioeng. Symp. 13, 113–120.
Kargi, F, Curme, J.A., and Sheehan, J.J. (1985a). Biotechnol Bioeng. 27, 34–40.
Kargi, F., and Curme, J.A. (1985b). Biotechnol Bioeng. 27, 1122–1125.
Seaman, J.F., Bubl, J.L. and Harris, E.E. (1945). Ind. Eng. Chem. Anal. Ed., 17, 35–37.
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Lezinou, V., Christakopoulos, P., Kekos, D. et al. Simultaneous saccharification and fermentation of sweet sorghum carbohydrates to ethanol in a fed-batch process. Biotechnol Lett 16, 983–988 (1994). https://doi.org/10.1007/BF00128638
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DOI: https://doi.org/10.1007/BF00128638