Summary
Pseudo-continuous culture of recombinant marine cyanobacteria was carried out under a light dark cycle. As a model foreign gene, the structural gene of chloramphenicol acetyl transferase (CAT) was used. Under a light dark alternate conditions, a steady state cell concentration was observed. However, the level of CAT expression did not show a stationary phase, but oscillated with the light dark cycle. This may be due to the lack of energy for foreign peptide synthesis during dark incubation. This result also indicates the possibility of regulation of foreign gene expression by controlling light illumination.
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Sode, K., Hatano, N. & Tatara, M. Pseudo-continuous culture of marine recombinant cyanobacteria under a light dark cycle. Biotechnol Lett 16, 973–976 (1994). https://doi.org/10.1007/BF00128636
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DOI: https://doi.org/10.1007/BF00128636