Summary
The effect of medium composition and initial glucose concentration on production of hEGF by recombinant E. coli cells was investigated. Optimum hEGF production was observed in a yeast extract/acid hydrolysed casein/salts media containing an initial glucose concentration of 10 g.l-1. A maximum hEGF titer of 250 mg.l-1 was obtained in this medium after 32 h in laboratory fermenters with pH, temperature, agitation and aeration set at 6.8, 30°C, 500 rpm and 2 vvm, respectively.
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Yadwad, V.B., Wilson, S. & Ward, O.P. Production of human epidermal growth factor by an ampicillin resistant recombinant Escherichia coli strain. Biotechnol Lett 16, 885–890 (1994). https://doi.org/10.1007/BF00128619
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DOI: https://doi.org/10.1007/BF00128619