Skip to main content
SpringerLink
Log in

Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture of Alcaligenes SP. SH-69

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate [poly(3HB-co-3HV)] by fed-batch culture of Alcaligenes sp. SH-69 was investigated using glucose as a sole carbon source. Synthesis of poly(3HB-co-3HV) during the polymer accumulation stage was favored under dissolved oxygen tension at 20% and C/N ratio (mol glucose/mol ammonium) of 23.1. When conditions were optimal, 36 g liter-1 of poly(3HB-co-3HV) containing 3.0 mol% of 3HV was produced. Decreasing C/N ratio resulted in an increase of 3HV fraction in the copolymer to a maximum level of 6.3 mol%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson,A. J. and Dawes,E.A.(1990) Microbiol. Rev. 54, 450–472

    Google Scholar 

  • Anderson,A.J., Haywood,G.W., Williams,D.R., and Dawes, E.A. (1990) in: Novel Biodegradable Microbial Polymers (ed. Dawes, E-A.) pp. 119–129, Kluwer Academic Publishers

  • Bitar,A. and Underhill,S. (1990) Biotechnol. Lett. 12, 563–568

    Google Scholar 

  • Bluhm,T.L., Hamer,G.K., Marchessault,R.H., Fyfe,C.A., and Veregin,R.P. (1986) Macromolecules 19, 2871–2876

    Google Scholar 

  • Braunegg,G., Sonnleitner,B., and Lafferty,R.M. (1978) Eur. J. Appl. Microbiol. Biotechnol. 6, 29–37

    Google Scholar 

  • Byrom,D. (1987) Trends Biotechnol. 5, 339–345

    Google Scholar 

  • Doi,Y., Kunioka,M., Nakamura,Y., and Soga,K. (1986) Macromolecules 19, 2860–2864

    Google Scholar 

  • Doi,Y., Segawa,A., and Kunioka,M. (1990) Int. J. Biol. Macromol. 12, 106–111

    Google Scholar 

  • Haywood,G.W., Anderson,A.J., and Dawes,E.A. (1989) Biotechnol. Lett. 11, 471–476

    Google Scholar 

  • Haywood,G.W., Anderson,A.J., Williams,D.R., and Dawes,E.A. (1991) Int. J. Biol. Macromol. 13, 83–88

    Google Scholar 

  • Holmes,P.A. (1985) Phys. Technol. 16, 32–36

    Google Scholar 

  • Kim,G.J., Yun,K.Y., Bae,K.S., and Rhee,Y.H. (1992) Biotechnol. Lett. 14, 27–32

    Google Scholar 

  • Ramsay,B.A., Lomaliza,K., Chavarie,C., Dube,B., Bataille,P., and Ramsay,J. (1990) Appl. Environ. Microbiol. 56, 2093–2098

    Google Scholar 

  • Rhee,Y.H., Jang,J-H, and Rogers,P.L. (1992) Aust. Biotechnol. 2, 230–232

    Google Scholar 

  • Senior,P.J., Beech,G.A., Ritchie,A.F., and Dawes,E.A. (1972) Biochem. J. 128, 1193–1201

    Google Scholar 

  • Shimizu, H., Sonoo, S., Shioya, S., and Suga, K.(1992) in: Biochemical Engineering for 2001 (eds. Furusaki, S., Endo, I. and Matsuno, R.) pp. 195–197, Springer-Verlag.

  • Sonnleitner,B., Heinzle,E., Braunegg,G., and Lafferty,R.M. (1979) Eur. J. Microbiol. Biotechnol. 7, 1–10

    Google Scholar 

  • Steinbüchel,A. and Pieper,U. (1992) Appl. Microbiol. Biotechnol. 37, 1–6

    Google Scholar 

  • Steinbüchel,A. and Schlegel,H.G. (1989) Appl. Microbiol. Biotechnol. 31, 168–175

    Google Scholar 

  • Suzuki,T., Yamane,T., and Shimizu,S. (1986a) Appl. Microbiol. Biotechnol. 23, 322–329

    Google Scholar 

  • Suzuki,T., Yamane,T., and Shimizu,S. (1986b) Appl. Microbiol. Biotechnol. 24, 366–369

    Google Scholar 

  • Suzuki,T., Yamane,T., and Shimizu,S. (1986c) Appl. Microbiol. Biotechnol. 24, 370–374

    Google Scholar 

  • Tal,S. and Okon,Y. (1985) Can. J. Microbiol. 31, 608–613

    Google Scholar 

  • Tombolini,R. and Nuti,M.P. (1989) FEMS Microbiol. Lett. 60, 299–304.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of New South Wales, 2033, Kensington, NSW, Australia

    Y. H. Rhee, J-H Jang & P. L. Rogers

  2. Department of Microbiology, Chungnam National University, 305-764, Daejeon, Korea

    Y. H. Rhee

Authors
  1. Y. H. Rhee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J-H Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. L. Rogers
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rhee, Y.H., Jang, JH. & Rogers, P.L. Production of copolymer consisting of 3-hydroxybutyrate and 3-hydroxyvalerate by fed-batch culture of Alcaligenes SP. SH-69. Biotechnol Lett 15, 377–382 (1993). https://doi.org/10.1007/BF00128280

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00128280

Keywords

Search

Navigation