Summary
Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell growth abilities.
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Shinmoto, H., Dosako, S. Adaptation of mouse-human hybridomas to a protein free medium. Biotechnol Lett 15, 327–330 (1993). https://doi.org/10.1007/BF00128270
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DOI: https://doi.org/10.1007/BF00128270