Skip to main content
SpringerLink
Log in

A highly regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using specific 1,2-α-mannosidase from Aspergillus phoenicis

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

A specific 1,2-α-mannosidase was isolated from A. phoenicis and used in the equilibrium-controlled synthesis of α1→2-linked mannobiose and mannotriose. Yields of 22.33 % disaccharide, 8.21 % trisaccharide and 2.74 % tetrasaccharide were obtained. Regioselectivity was absolute for the 1→2 linkage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ciucanu, I. and Kerek, F. 1984. Carbohydrate Research, 131, 209–217.

    Google Scholar 

  • Harris, P.J., Henry, R.J., Blakeney, A.B., and Stone, B.A. 1984. Carbohydrate Research, 127, 59–73.

    Google Scholar 

  • Ichishima, E., Arai, M., Shigematsu, Y., Kumagai, H., and Sumida-Tanaka, R. (1981). Biochim. Biophys. Acta, 658, 45–53.

    Google Scholar 

  • Johansson, E., Hedbys, L., Mosbach, K., Larsson, P.-O., Gunnarsson, A., and Svensson, S. 1989. Enzyme Microbial Technol., 11, 347–352.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., 1951. J. Biol. Chem., 193, 265–275.

    Google Scholar 

  • Raper, K.B. and Fennel, D.I. 1965. The genus: Aspergillus. The Williams and Wilkins Comp. Baltimore, USA, 293–338.

    Google Scholar 

  • Rastall, R.A. and Bucke, C. 1992. Biotechnology and Genetic Engineering Reviews, 10, 253–281.

    Google Scholar 

  • Rastall, R.A., Rees, N.H., Wait, R., Adlard, M.W., and Bucke, C. 1992. Enzyme Microbial Technol., 14, 53–57.

    Google Scholar 

  • Somogyi-Nelson, M. 1952. J. Biol. Chem., 195, 19–23.

    Google Scholar 

  • Yamashita,K. and Ichishima, E. 1980. Biochemical and Biophysical Research Communications, 96, 1335–1342.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biotechnology and Biochemical Engineering Group, Department of Food Science and Technology, University of Reading, Whiteknights, P.O. Box 226, RG6 6AP, Reading, UK

    Sony Suwasono & Robert A. Rastall

Authors
  1. Sony Suwasono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert A. Rastall
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suwasono, S., Rastall, R.A. A highly regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using specific 1,2-α-mannosidase from Aspergillus phoenicis . Biotechnol Lett 18, 851–856 (1996). https://doi.org/10.1007/BF00127901

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00127901

Keywords

Search

Navigation