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Characterisation of 2,5-diketo-D-gluconic acid reductase from Corynebacterium sp.

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Summary

2,5-diketo-D-gluconic acid reductase, that converts 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid (the direct precursor of vitamin C) was extracted and purified from Corynebacterium sp.. The enzyme was characterised in terms of kinetic parameters, molecular weight and isoelectric point. Enzyme stability at different operating temperatures was investigated, as well.

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Authors and Affiliations

  1. Dipartimento di Ingegneria Chimica, Università di Napoli Federico II, Piazzale V. Tecchio 80, 80125, Napoli, Italy

    Michele Maremonti & Guido Greco jr

  2. Arbeitsgruppe Bioverfahrenstechnik, Fachbereich Chemietechnik, Universität Dortmund, Emil-Figge Straβe 68, D-44227, Dortmund, Germany

    Rolf Wichmann

Authors
  1. Michele Maremonti
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  2. Guido Greco jr
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  3. Rolf Wichmann
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Maremonti, M., Greco, G. & Wichmann, R. Characterisation of 2,5-diketo-D-gluconic acid reductase from Corynebacterium sp. . Biotechnol Lett 18, 845–850 (1996). https://doi.org/10.1007/BF00127900

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  • DOI: https://doi.org/10.1007/BF00127900

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