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Characteristics of phospholipase D in reverse micelles of Triton X-100 and phosphatidylcholine in diethyl ether

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Summary

Phospholipase D, from cabbage, is active in reverse micelles formed from its substrate phosphatidylcholine and Triton X-100 in diethyl ether. The activity is optimum at w0=12.5. The increase of the molar ratio of Triton X-100/substrate from 1:4 to 2:1 results in an activity decrease by 25 %. At 136 mM Triton X-100 the KM value in reverse micelles is 136 mM, whereas it is 0.40 mM in the aqueous system containing SDS.

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Authors and Affiliations

  1. Department of Biochemistry/Biotechnology, Martin-Luther University, D-06099, Halle, Federal Republic of Germany

    S. Subramani, N. Dittrich, F. Hirche & R. Ulbrich-Hofmann

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  1. S. Subramani
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  2. N. Dittrich
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  3. F. Hirche
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  4. R. Ulbrich-Hofmann
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Subramani, S., Dittrich, N., Hirche, F. et al. Characteristics of phospholipase D in reverse micelles of Triton X-100 and phosphatidylcholine in diethyl ether. Biotechnol Lett 18, 815–820 (1996). https://doi.org/10.1007/BF00127895

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  • DOI: https://doi.org/10.1007/BF00127895

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