Summary
A new thermophilic Streptomyces sp. TO1, isolated from Tunisian soil, produced a thermostable alpha-amylase and pullulanase. The gene encoding for the alpha-amylase activity was cloned into the multicopy cloning plasmid pLM1 using S. lividans ZX1 as host strain. The ZX1 / pLM1 strain has the same activity than the initial TO1 strain and about 25 fold higher activity than the ZX1 strain. This alpha-amylase has an optimum of pH and temperature at 6 and 70 °C respectively.
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Mellouli, L., Ghorbel, R., Kammoun, A. et al. Characterization and molecular cloning of thermostable alpha-amylase from Streptomyces sp.To1. Biotechnol Lett 18, 809–814 (1996). https://doi.org/10.1007/BF00127894
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DOI: https://doi.org/10.1007/BF00127894