Abstract
A new approach for the highly specific preparation of L-serine conjugates of lactosamine and Galβ1-3GalNAc is described. Thus, the L-serine derivative of lactosamine Galβ1-4GlcNAcβ-O-(N-Z)-Ser-OEt, was obtained from lactose, employing GlcNAcβ-O-(N-Z)-Ser-OEt as acceptor and a yeast β-galactosidase as catalyst Galp 1-3GalNAcα-O-(N-Alloc)-Ser-OMe was obtained from lactose, employing GalNAcα-O-(N-Alloc)-Ser-OMe as acceptor and β-galactosidase from bovine testes as catalyst.
Similar content being viewed by others
References
Bevilacqua, M. (1993). Annu. Rev. Immunol. 11, 767–790.
Cantacuzene, D. and Attal, S. (1991). Carbohydr. Res. 211, 327–331.
Feizi, T. (1993). Curr. Opin. Struct. Biol. 3, 701–710.
Johansson, E., Hedbys, L. and Larsson, P.-O. (1991). Enzyme Microb. Technol. 13, 781–787.
Karlsson, K.A. (1989). Annu. Rev. Biochem. 58, 309–350.
Nilsson, K.G.I. (1988). Trends Biotechnol. 6, 256–264.
Nilsson, K.G.I. and Scigelová, M (1994). Biotechnol. Lett. 16, 671–676.
Nilsson, K.G.I., Eliasson, A. and Lorek, U. (1995). Biotechnol. Lett. 17, 717–722.
Nilsson, K.G.I. (1996). Synthesis with Glycosidases. In: Modern Methods in Carbohydrate Synthesis, S.H. Khan and R.A. O'Neill, eds. pp. 518–547, Amsterdam: Harwood Academic Publishers.
Peters, S., Meldal, M. and Bock, K. (1996). Recent developments in Glycopeptide Synthesis. In: Modern Methods in Carbohydrate Synthesis, S.H. Khan and R.A. O'Neill, eds. pp. 352–377, Amsterdam: Harwood Academic Publishers.
Schultz, M. and Kunz, H. (1993). Tetrahedron Asymmetry 4, 1205–1220.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nilsson, K.G.I. Synthesis of Galβ1-4GlcNAcβ- and Galβ1-3GalNAcα-O-L-serine Derivatives employing glycosidases. Biotechnol Lett 18, 791–794 (1996). https://doi.org/10.1007/BF00127890
Issue Date:
DOI: https://doi.org/10.1007/BF00127890