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Glutamine is not an essential amino acid for Sf-9 insect cells

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Summary

Spodoptera frugiperda (Sf-9) insect cells are fully capable of growth and proliferation in a glutamine, glutamate and aspartate-free medium, provided that ammonium ions are supplied. S. frugiperda (Sf-21) and Mamestra brassicae cells (IZD-MB-0503) also grow in glutamine-free media but not Trichoplusia ni cells (BTI-TN 5B1-4). The yield of β-galactosidase in Sf-9 cells infected with a recombinant baculovirus under glutamine-free conditions was even higher than the yield obtained in glutamine containing cultures.

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References

  • Bédard, C., Tom, R. and Kamen, A. (1993). Biotechnol. Prog. 9, 615–624.

    Google Scholar 

  • Bray, H.G., James, S.P., Raffan, I.M. and Thorpe, W.V. (1949). Biochem. J. 44, 625–627.

    Google Scholar 

  • Ferrance, J.P., Goel, A. and Ataai, M.M. (1993). Biotechnol. Bioeng. 42, 697–707.

    Google Scholar 

  • Kamen, A.A., Tom, R.L., Caron, A.W., Chavarie, C., Massie, B. and Archambault, J. (1991). Biotechnol. Bioeng. 38, 619–628.

    Google Scholar 

  • Miltenburger, H.G. and David, P. (1980). Develop. biol. Standard, 46, 183–186.

    Google Scholar 

  • Mitsuhashi, J. (1982a). Media for insect cell cultures. In: Advances in cell Culture, K. Maramorosch, ed. vol. 2. pp. 133–196, New York: Academic Press, Inc.

    Google Scholar 

  • Mitsuhashi, J. (1982b). Determination of essential amino acids for insect cell lines In: Invertebrate Cell Culture Applications, pp. 9–51, New York: Academic Press, Inc.

    Google Scholar 

  • Reuveny, S., Kim, Y.J., Kemp, C.W. and Shiloach, J. (1993). Appl. Microbiol. Biotechnol. 38, 619–623.

    Google Scholar 

  • Tritsch, G.A. and Moore, G.E. (1962). Exp. Cell. Res. 28, 360–364.

    Google Scholar 

  • Wang, M.-Y., Kwong, S. and Bentley, W.E. (1993). Biotechnol. Prog. 9, 355–361.

    Google Scholar 

  • Öhman, L., Ljunggren, J. and Häggström, L. (1995). Appl. Microbiol. Biotechnol. 43, 1006–1013.

    Google Scholar 

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Authors and Affiliations

  1. KaroBio AB, S-141 57, Novum, Huddinge, Sweden

    Lars Öhman, Maria Alarcon, Jan Ljunggren & Anna-Karin Ramqvist

  2. Department of Biochemistry and Biotechnology, Royal Institute of Technology, S-100 44, Stockholm, Sweden

    Lena Häggström

Authors
  1. Lars Öhman
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  2. Maria Alarcon
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  3. Jan Ljunggren
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  4. Anna-Karin Ramqvist
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  5. Lena Häggström
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Öhman, L., Alarcon, M., Ljunggren, J. et al. Glutamine is not an essential amino acid for Sf-9 insect cells. Biotechnol Lett 18, 765–770 (1996). https://doi.org/10.1007/BF00127885

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  • DOI: https://doi.org/10.1007/BF00127885

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