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Penicillin acylases as amidohydrolases and acyl transfer catalysts

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Summary

The hypothesis that an acyl-enzyme intermediate of the esterases from Xanthomonas citri and Acetobacter turbidans is reponsible for the acylation of 7-aminodeacetoxycephalosporanic acid and similar compounds by esters of amino acids, recently proposed by Kato, also accounts for the kinetics of hydrolysis of penicillin G by the amidohydrolase from E. coli and other reactions of the enzyme. Some further implications of the mechanism are derived and discussed.

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Authors and Affiliations

  1. Pharmaceuticals Division, CIBA-GEIGY Limited, CH-4002, Basle, Switzerland

    J. Konecny

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  1. J. Konecny
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This paper is dedicated to Prof. G. Manecke, a pioneer of immobilized enzyme technology, with congratulations to his 65th birthday.

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Konecny, J. Penicillin acylases as amidohydrolases and acyl transfer catalysts. Biotechnol Lett 3, 112–117 (1981). https://doi.org/10.1007/BF00127361

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  • DOI: https://doi.org/10.1007/BF00127361

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