Summary
A new immobilization method is described using the ionotropic gelation of a polycation (chitosan) with different multivalent anionic counterions. Immobilized E-coli cells with tryptophan synthetase show ~ 57 % of the initial activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bang, W.G., Lang, S., Sahm, H., and Wagner, F. (1978) Prepr.-Eur. Congr. Biotechnol. 1 st, 186–189
Brodelius, P., Deus, B., Mosbach, K. and Zenk, M.H. (1979), FEBS Lett. 103(1), 93–97
Chibata, I., and Tosa, T. (1977) Adv. Appl. Microbiol. 22, 1–27
Durand, G. and Navarro, J,M, (1978) Process Biochemistry 13(9), 14–23
Hackel, U., Klein, J., Megnet, R., and Wagner, F. (1975), Europ. J. Appl.Microbiol. 1, 291–293
Kierstan, M., and Bucke, C. (1977) Biotechnol. Bioeng. 19, 387–397
Klein, J., and Wagner, F. (1978) Dechema-Monograph. Vol.82, 142–164, Vlg.-Chemie, Weinheim
Klein, J., and Wagner, F. (1979a) Dechema-Monograph. Vol.84, 265–335, Vlg.-Chemie, Weinheim
Klein, J., Hackel, U., and Wagner, F. (1979b) ACS Sympos. Series 106, 101–118
Ohlson, S., Larson, P.O., and Mosbach, K. (1979) Eur. J. Appl. Microbiol. Biotechnol. 7, 103–110
Pilwat, G., Washausen, P., Klein, J., and Zimmermann (1980), Z. Naturforsch. 35c, 352–356
Scheurich, P., Schnabl, H., Zimmermann, U., and Klein, J. (1980) Biochim. Biophys. Acta 598, 645–651
Vorlop, K.-D., Lang, S., Klein, J., Wagner, F. (1981) Eur. Congr. Biotechnol. 2 nd , in press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vorlop, KD., Klein, J. Formation of spherical chitosan biocatalysts by ionotropic gelation. Biotechnol Lett 3, 9–14 (1981). https://doi.org/10.1007/BF00127253
Issue Date:
DOI: https://doi.org/10.1007/BF00127253