Skip to main content
SpringerLink
Log in

Morphological analysis of metastatic potential and antimetastatic drug effects in mice bearing two lines of Lewis lung carcinoma

  • Published:
Clinical & Experimental Metastasis Aims and scope Submit manuscript

Two lines of Lewis lung carcinoma with a different potential to metastasize spontaneously to the lungs have been examined for their cytological and histological characteristics. Metastatic potential appears to be related with parenchymal organization of the primary tumours, since large haemorrhagic areas containing detached tumour cells and the absence of endothelialized capillaries are observed only in the line with high metastatic potential. At the same time, the cytological characteristics of the cells of the two tumour lines are similar, and do not seem to be related with metastatic potential. After treatment with the selective antimetastatic drugs ICRF-159 and DM-COOK, and with DTIC, the histological appearance of the line with high metastatic potential becomes similar to that of the line with low metastatic potential. These data seem to indicate that the early phases of tumour spread occurring in the primary tumour are of relevance for metastatic potential and control by antimetastatic drugs, and suggest that for DTIC such antimetastatic action may participate to its clinical antitumour effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fidler, I. J., 1984, Recent concepts of cancer metastasis and their implications for therapy. Cancer Treatment Reports, 68, 193–198.

    Google Scholar 

  2. [2]Giraldi, T., Sava, G., Cuman, R., Nisi, C., and Lassiani, L., 1981, Selectivity of the antimetastatic and cytotoxic effects of 1-p-(3,3-dimethyl-1-triazeno)benzoic acid potassium salt, (±)-1,2-di(3,5-dioxopiperazin-1-yl)propane, and cyclophosphamide in mice bearing Lewis lung carcinoma. Cancer Research, 41, 2524–2528.

    Google Scholar 

  3. Giraldi, T., Sava, G., Cherubino, R., Bottiroli, G., and Mazzini, G., 1984, Effects of DTIC, DM-COOK and ICFR-159 on the number of circulating Lewis lung carcinoma cells detected by flow cytometry. Clinical and Experimental Metastasis, 2, 151–159.

    Google Scholar 

  4. James, S. E., and Salsbury, A. J., 1974, Effect of (±)-1,2-bis(3,5-dioxopiperazin-l-yl)propane on tumor blood vessels and its relationship to the antimetastatic effect in the Lewis lung carcinoma. Cancer Research, 34, 839–842.

    Google Scholar 

  5. Sacchi, A., Corsi, A., Caputo, M., and Zupi, G., 1979, In vitro and in vivo selection of two Lewis lung carcinoma cell lines. Tumori, 65, 657–664.

    Google Scholar 

  6. Salsbury, A. J., Burrage, K., and Hellmann, K., 1970, Inhibition of metastatic spread by ICRF 159: selective deletion of malignant characteristics. British Medical Journal, 4, 344–346.

    Google Scholar 

  7. Salsbury, A. J., Burrage, K., and Hellmann, K., 1974, Histological analysis of the antimetastatic effects of (±)-1,2-bis(3,5-dioxopiperazin-1-yl)propane. Cancer Research, 34, 843–849.

    Google Scholar 

  8. Sato, T., Takusagawa, K., Asoo, N., Ariji, F., Shida, K., Kumano, N., and Konno, K., 1982, Ultrastructure of the Lewis lung carcinoma. European Journal of Cancer and Clinical Oncology, 18, 369–376.

    Google Scholar 

  9. Sava, G., Giraldi, T., Zupi, G., and Sacchi, A., 1984, Effects of antimetastatic dimethyltriazenes in mice bearing Lewis lung carcinoma lines with different metastatic potential. Invasion and Metastasis, 4, 171–178.

    Google Scholar 

  10. Starace, G., Badaracco, G., Greco, G., Sacchi, A., and Zupi, G., 1982, DNA content distribution of in vivo and in vitro lines of Lewis lung carcinoma. European Journal of Cancer and Clinical Oncology, 18, 973–978.

    Google Scholar 

  11. Zamboni, L., and De Martino, C., 1967, Buffered picric acid-formaldehyde: a new rapid fixative for electron microscopy. Journal of Cell Biology, 35, 148A.

  12. Zupi, G., Mauro, F., and Sacchi, A., 1980, Cloning in vitro and in vivo of Lewis lung carcinoma, properties and characteristics. British Journal of Cancer, 41, Suppl. 4, 309–310.

    Google Scholar 

  13. Zupi, G., Cavallari, A., Greco, C., and Sacchi, A., 1984, Lewis lung carcinoma lines with different metastatic behaviour: response to ICRF-159. Anticancer Research, 4, 91–95.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Istituto di Anatomia Umana Normale, Università di Trieste, I-34100, Trieste, Italy

    Vittorio Grill & Franco Mallardi

  2. Istituto di Farmacologia, Università di Trieste, I-34100, Trieste, Italy

    Sonia Zorzet, Laura Perissin & Tullio Giraldi

Authors
  1. Vittorio Grill
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Franco Mallardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sonia Zorzet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Laura Perissin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tullio Giraldi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grill, V., Mallardi, F., Zorzet, S. et al. Morphological analysis of metastatic potential and antimetastatic drug effects in mice bearing two lines of Lewis lung carcinoma. Clin Exp Metast 5, 233–244 (1987). https://doi.org/10.1007/BF00124305

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00124305

Keywords

Search

Navigation