Sex-chromosomes in a male and a female clone of M. dioicum were studied by fluorescent staining with quinacrine-mustard. A dark band was found in the middle of the long arm of one X-chromosome in the female. In the other X-chromosome no band was visible, but a slightly enhanced fluorescence was shown in the same region. In the Y-chromosome a more intense fluorescence was found on both ends of the chromosome and a slight overall difference between the arms.
The relation between fluorescence pattern and heterochromatin is discussed.
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Adkisson, K. P., W. J. Perreault & H. Gay (1971). Differential fluorescent staining of Drosophila chromosomes with quinacrine mustard. Chromosoma 34: 190–205.
Caspersson, T., L. Zech, E. H. Folev, U. Wagh & E. Simonsson (1969). Chemical differentiation with fluorescent alkylating agents in Vicia faba metaphase chromosomes. Exp. Cell Res. 58: 128–140.
Caspersson, T., G. Lomakka & L. Zech (1971). The 24 fluorescence patterns of the human metaphase chromosomes—distinguishing characters and variability. Hereditas 67: 89–102.
Choudhuri, H. C. (1969). Late DNA replication pattern in sex chromosomes of Melandrium. Can. J. Genet. Cytol. 11: 192–198.
Comings, D. E. (1971). Heterochromatin of the Indian Muntjac. Exp. Cell Res. 67: 441–460.
Darlington, C. D. (1965). Cytology. J. & A. Churchill Ltd., London.
Darlington, C. D. & La Cour (1940). Nucleic acid starvation of chromosomes in Trillium. J. Genet. 40: 185–213.
Fredga, K. (1971). Idiogram and fluorescence pattern of the chromosomes of the Indian muntjac. Hereditas 68: 332–337.
Ganner, E. & H. J. Evans (1971). The relationship between patterns of DNA-replication and of quinacrine fluorescence in the human chromosome complement. Chromosoma 35: 326–341.
Heitz, E. (1928). Das Heterochromatin der Moose. Jahrb. Wiss. Bot. 69: 762–818.
Löve, D. (1944). Cytogenetic studies on dioccious Melandrium. Botan. Notiser (1944): 125–214.
Nigtevecht, G.van (1966). Genetic studies in dioecious Melandrium. II: Sex determination in Melandrium album and Melandrium dioicum. Genetica 37: 307–344.
Vosa, C. G. (1970a). Heterochromatin recognition with fluorochromes. Chromosoma 80: 366–372.
Vosa, C. G. (1970b). The discriminating fluorescence patterns of the chromosomes of Drosophila. Chromosoma 31: 446–451.
Vosa, C. G. (1971). The quinacrine-fluorescence patterns of the chromosomes of Allium carinatum. Chromosoma 33: 382–385.
Warmke, H. E. (1946). An analysis of male development in Melandrium by means of Y-chromosome deficiencies. Genetics 31: 234–235.
Westergaard, M. (1946). Aberrant Y-chromosomes and sex expression in Melandrium album. Hereditas 32: 419–443.
Westergaard, M. (1948). The relation between chromosome constitution and sex in the offspring in triploid Melandrium. Hereditas 34: 257–279.
Westergaard, M. (1958). The mechanism of sex determination in dioecious flowering plants. Adv. Genet. 9: 217–281.
Zech, L. (1969). Investigation of metaphase chromosomes with DNA-binding fluorochromes. Exp. Cell Res. 58: 463.
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Kampmeijer, P. Fluorescence pattern of the sex-chromosomes of Melandrium dioicum, stained with quinacrine-mustard. Genetica 43, 201–206 (1972). https://doi.org/10.1007/BF00123626
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DOI: https://doi.org/10.1007/BF00123626