Following disc electrophoresis on standard gels, rye seed extracts showed two bands (ADH-3 and 5) for alcohol dehydrogenase. The ADH-3 band was homologous to the ADH band observed in other diploid species of the Triticinae, and with the ADH-3 band of 4 × and 6 × wheat. It is proposed that the rye isoenzymes ADH-3 and 5 are governed respectively, by the genes Adh R1 and Adh R2. Using bread wheat (Holdfast) lines with disomic addition of individual rye (King II) chromosomes, we found that the ADH-5 band was associated with the addition of rye chromosome IV (after Riley), indicating thereby that Adh R2 gene is located on this chromosome. The products of Adh R1 and Adh R2 do not form active heterodimers, among themselves, but do form active dimers with wheat ADH monomers. It is suggested that the use of chromosomal addition lines may provide a method for locating genes for those enzymes, where the rye and wheat isoenzymes are electrophoretically distinct.
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References
Barber, H. N., C. J. Driscoll & R. S. Vickerv (1968). Enzymic markers for wheat and rye chromosomes. Proc. IIIrd Int. Wheat Genet. Symp. (Austral. Acad. Sci., Canberra), p. 116.
Bhatia, C. R. & H. H. Smith (1966). Variation associated with an Aegilops umbellulata chromosome segment incorporated in wheat. Nature 211: 1425–1426.
Brewer, G. J. (1970). An introduction to isoenzyme techniques. Academic Press, New York.
Brewer, G. J., C. F. Sing & E. R. Sears (1969). Studies of isozyme patterns in nullisomic-tetrasomic combinations of hexaploid wheat. Proc. natn. Acad. Sci. U.S.A. 64: 1224–1229.
Gupta, P. K. (1971). Homocologous relationship between wheat and rye chromosomes. Present status. Genetica 42: 199–213.
Hart, G. E. (1970). Evidence for triplicate genes for ADH in hexaploid wheat. Proc. natn. Acad. Sci. U.S.A. 66: 1136–1141.
Mitra, R. & C. R. Bhatia (1971). Isoenzymes and polyploidy I: Qualitative and quantitative isoenzyme studies in the Triticinae. Genet. Res. 18: 57–69.
O'Mara, J. G. (1946). The substitution of a specific Secale cereale chromosome for a specific Triticum vulgare chromosome, Rec. Genet. Soc. Am. 15: 62.
Rilev, R. (1965). Cytogenetics and plant breeding. Genetics Today 3: 681.
Rilev, R. & G. Kimber (1966). The transfer of alien genetic variation to wheat. Report Plant Breeding Institute Cambridge 1964–65: 6–36.
Sears, E. R. (1968). Relationship of chromosome 2A, 2B and 2D with their rye homoeologue. Proc. IIIrd Int. Wheat Genet. Symp. Canberra, pp. 53–61.
Sears, E. R., (1969), Wheat cytogenetics, Ann. Rev. Genet. 3: 451–468.
Yee, Y. H., E. N. Larter & L. E. Evans (1969). Homoeologous relationship of rye chromosome VI (3R) with two homoeologous groups from wheat. Canad. J. Genet. Cytol. 11: 803–809.
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Irani, B.N., Bhatia, C.R. Chromosomal location of alcohol dehydrogenase gene(s) in rye, using wheat-rye addition lines. Genetica 43, 195–200 (1972). https://doi.org/10.1007/BF00123625
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DOI: https://doi.org/10.1007/BF00123625