Abstract
We report a simplified method modified from standard procedures for the production of long-term primary skeletal muscle monolayer cell cultures using collagenous for tissue digestion. When grown on the commercially available substrate Matrigel, such cultures are high in myotube content, remain attached to the plate surface after the initiation of spontaneous activity and do not need to be treated with mitotic inhibitors to control fibroblast proliferation. In addition cultures even more enriched for myotubes can be produced by selective removal of fibroblasts from Matrigel coated plates by collagenase. This novel procedure, along with the simplified primary culture technique, allows for highly reproducible results even for the inexperienced user.
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O'Malley, J.P., Greenberg, I. & Salpeter, M.M. The production of long-term rat muscle cell cultures on a Matrigel substrate and the removal of fibroblast contamination by collagenase. Methods Cell Sci 18, 19–23 (1996). https://doi.org/10.1007/BF00123519
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DOI: https://doi.org/10.1007/BF00123519