Skip to main content
Log in

Silver staining of nucleolar organizers and nucleoli during mitosis and interphase of mammalian cells

  • Published:
Genetica Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Anastassova-Kristeva, M. (1977). The nucleolar cycle in man. J. Cell Sci. 25: 103–110.

    Google Scholar 

  • Anastassova-Kristeva, M., H.Nicoloff, G.Künzel & R.Rieger (1977). Nucleolus formation in structurally reconstructed barley karyotypes with six satellite chromosomes. Chromosoma 62: 111–117.

    Google Scholar 

  • Barr, H.J. & H.Esper (1963). Nucleolar size in cells of Xenopus laevis in relation to nucleolar competition. Expl Cell Res. 31: 211–214.

    Google Scholar 

  • Bloom, S.E. & C.Goodpasture (1976). An improved technique for selective staining of nucleolar organizer regions in human chromosomes. Hum. Genet. 34: 199–206.

    Google Scholar 

  • Branch, A.D. & M.W.Berns (1976). Nucleoli and ploidy in Potorous cells (PTK2) in vitro. Chromosoma 56: 33–40.

    Google Scholar 

  • Díez, J.L., M.F.Marín, P.Esponda & J.C.Stockert (1971). Prenucleolar bodies in the cytoplasm of meristematic cells after thermal shock. Experientia 27: 266–267.

    Google Scholar 

  • Estable, C. (1966). Morphology, structure, and dynamics of the nucleolonema. Natn. Cancer Inst. Monogr. 23: 91–105.

    Google Scholar 

  • Estable, C. & J.R.Sotelo (1955). The behaviour of the nucleolonema during mitosis. In: Symp. Fine Structure of Cells. Groningen, Noordhoff, p. 170–190.

    Google Scholar 

  • Evans, H.J., R.A.Buckland & M.L.Pardue (1974). Location of genes coding for 18S and 28S ribosomal RNA in the human genome. Chromosoma 48: 405–426.

    Google Scholar 

  • Feinendegen, L.E. & V.P.Bond (1963). Observations on nuclear RNA during mitosis in human cancer cells in culture (HeLa-S3), studied with tritiated cytidine. Expl Cell Res. 30: 393–404.

    Google Scholar 

  • Ghosh, S. (1976). The nucleolar structure. Int. Rev. Cytol. 44: 1–28.

    Google Scholar 

  • Ghosh, S., R.Lettré & I.Ghosh (1969). On the composition of the nucleolus with special reference to its filamentous structure. Z. Zellforsch. mikrosk. Anat. 101: 254–265.

    Google Scholar 

  • Goessens, G. & A.Lepoint (1974). The fine structure of the nucleolus during interphase and mitosis in Ehrlich tumour cells cultivated in vitro. Expl Cell Res 87: 63–72.

    Google Scholar 

  • Goodpasture, C. & S.E.Bloom (1975). Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53: 37–50.

    Google Scholar 

  • Goodpasture, C., S.E.Bloom, T.C.Hsu & F.E.Arrighi (1976). Human nucleolus organizers: The satellites or the stalks? Am. J. hum. Genet. 28: 559–566.

    Google Scholar 

  • Hayata, I., M.Oshimura & A.A.Sandberg (1977). N-band polymorphism of human acrocentric chromosomes and its relevance to satellite association. Hum. Genet. 36: 55–61.

    Google Scholar 

  • Heitz, E. (1931). Die Ursache der gesetzmässigen Zahl, Lage, Form und Grösse pflanzlicher Nukleolen. Planta 12: 775–844.

    Google Scholar 

  • Henderson, A.S., E.M.Eicher, M.T.Yu & K.C.Atwood (1976). Variation in ribosomal RNA gene number in mouse chromosomes. Cytogenet. Cell Genet. 17: 307–316.

    Google Scholar 

  • Heneen, W.K. (1976). HeLa cells and their possible contamination of other cell lines: Karyotype studies. Hereditas 82: 217–248.

    Google Scholar 

  • Heneen, W.K. (1978). Silver staining and nucleolar patterns in human heteroploid and measles-carrier cells. Hereditas 88: 213–217.

    Google Scholar 

  • Heneen, W.K. & W.Nichols (1966). Persistence of nucleoli in short term and long term cell cultures and in direct bone marrow preparations in mammalian materials. J. Cell Biol. 31: 543–561.

    Google Scholar 

  • Howell, W.M., T.E.Denton & J.R.Diamond (1975). Differential staining of the satellite regions of human acrocentric chromosomes. Experientia 31: 260–262.

    Google Scholar 

  • Hsu, T.C., F.E.Arrighi, R.R.Klevecz & B.R.Brinkley (1965). The nucleoli in mitotic divisions of mammalian cells in vitro. J. Cell Biol. 26: 539–553.

    Google Scholar 

  • Hsu, T.C., B.R.Brinkley & F.E.Arrighi (1967). The structure and behavior of the nucleolus organizers in mammalian cells. Chromosoma 23: 137–153.

    Google Scholar 

  • Hsu, T.C., S.E.Spirito & M.L.Pardue (1975). Distribution of 18+28S ribosomal genes in mammalian genomes. Chromosoma 53: 25–36.

    Google Scholar 

  • Hubbell, H.R. & T.C.Hsu (1977). Identification of nucleolus organizer regions (NORs) in normal and neoplastic human cells by the silver-staining technique. Cytogenet. Cell Genet. 19: 185–196.

    Google Scholar 

  • LaCour, L.F. (1966). The internal structure of nucleoli. Chromosomes Today 1: 150–160.

    Google Scholar 

  • Lafontaine, J.G. & A.Lord (1974). A correlated light- and electron-microscope investigation of the structural evolution of the nucleolus during the cell cycle in plant meristematic cells (Allium porrum). J. Cell Sci. 16: 63–93.

    Google Scholar 

  • Lettré, R., W.Siebs & N.Paweletz (1966). Morphological observations on the nucleolus of cells in tissue culture, with special regard to its composition. Natn. Cancer Inst. Monogr. 23: 107–123.

    Google Scholar 

  • Longwell, A.C. & G.Svihla (1960). Specific chromosomal control of the nucleolus and of the cytoplasm in wheat. Expl Cell Res. 20: 294–312.

    Google Scholar 

  • Lord, A. & J.G.Lafontaine (1969). The organization of the nucleolus in meristematic plant cells. A cytochemical study. J. Cell Biol. 40: 633–647.

    Google Scholar 

  • Matsui, S.-I. & M.Sasaki (1973). Differential staining of nucleolus organizers in mammalian chromosomes. Nature 248: 148–150.

    Google Scholar 

  • McClintock, B. (1934). The relation of a particular chromosomal element to the development of the nucleoli in Zea mays. Z. Zellforsch. mikrosk. Anat. 21: 294–328.

    Google Scholar 

  • Mikelsaar, A.-V., M.Schmid, W.Krone, H.G.Schwarzacher & W.Schnedl (1977a). Frequency of Ag-stained nucleolus organizer regions in the acrocentric chromosomes of man. Hum. Genet. 37: 73–77.

    Google Scholar 

  • Mikelsaar, A.-V., H.G.Schwarzacher, W.Sehnedl & P.Wagenbichler (1977b). Inheritance of Ag-stainability of nucleolus organizer regions. Investigations in 7 families with trisomy 21. Hum. Genet. 38: 183–188.

    Google Scholar 

  • Miller, L. & D.D.Brown (1969). Variation in the activity of nucleolar organizers and their ribosomal gene content, Chromosoma 28: 430–444.

    Google Scholar 

  • Miller, D.A., V.G.Dev, R.Tantravahi & O.J.Miller (1976a). Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Expl Cell Res. 101: 235–243.

    Google Scholar 

  • Miller, D.A., R.Tantravahi, V.G.Dev & O.J.Miller (1977), Frequency of satellite association of human chromosomes is correlated with amount of Ag-staining of the nucleolus organizer region. Am. J. hum. Genet. 29: 490–502.

    Google Scholar 

  • Miller, O.J., D.A.Miller, V.G.Dev, R.Tantravahi & C.M.Croce (1976b). Expression of human and suppression of mouse nucleolus organizer activity in mouse-human somatic cell hybrids. Proc. natn. Acad. Sci. U.S.A. 73: 4531–4535.

    Google Scholar 

  • Miller, O.L. & B.R.Beatty (1969). Visualization of nucleolar genes, Science 164: 955–957.

    Google Scholar 

  • Ohnuki, Y., R.S.Olson & D.E.Rounds (1972). Inhibition of nucleolar formation in Pt-K1 rat-kangaroo cells by laser microbeam irradiation. Jap. J. Genet. 47: 133–136.

    Google Scholar 

  • Pardue, M.L. & T.C.Hsu (1975). Locations of 188 and 28S ribosomal genes on the chromosomes of the Indian muntjac, J. Cell Biol. 64: 251–254.

    Google Scholar 

  • Schwarzacher, H.G., A.-V.Mikelsaar & W.Schnedl (1977). Silver-staining of nucleolus organizer regions in man: Light- and electron-microscopic observations. Chromosomes Today 6: 45–50.

    Google Scholar 

  • Smetana, K. & H.Busch (1974). The nucleolus and nucleolar DNA. In The Cell Nucleus Vol. 1 (Ed. H.Busch), Acad. Press, New York, p. 73–147.

    Google Scholar 

  • Stockert, J.C., J.M.Sogo, J.L.Díez & G.Giménez-Martín (1969). Alteration in the SAT-chromosome of Allium cepa affecting the size of the nucleolus. Experientia 25: 773–774.

    Google Scholar 

  • Vagner-Capodano, A.-M., M.-H.Binna-Delgrossi & A.Stahl (1977). Localization of nucleolar organizers in the interphase nucleus of human fibroblasts. Biol. Cell. 29: 209–210.

    Google Scholar 

  • Varley, J.M. (1977). Patterns of silver staining of human chromosomes. Chromosoma 61: 207–214.

    Google Scholar 

  • Warburton, D., K.C.Atwood & A.S.Henderson (1976). Variation in the number of genes for rRNA among human acrocentric chromosomes: Correlation with frequency of satellite association. Cytogenet. Cell Genet. 17: 221–230.

    Google Scholar 

  • Zankl, H., S.Stengel-Rutkowski & K.D.Zang (1973), The role of acrocentric chromosomes in nucleolar organization. II. Constancy of the nucleolar area independent of the number of acrocentric chromosomes in meningioma cell cultures. Virchows Arch. path. Anat. Physiol. Abt. B Zellpathol. 13: 113–118.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heneen, W.K. Silver staining of nucleolar organizers and nucleoli during mitosis and interphase of mammalian cells. Genetica 50, 31–39 (1979). https://doi.org/10.1007/BF00122676

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00122676

Keywords

Navigation