Skip to main content
Log in

Effects of the herbicide alachlor on soil microbial activities

  • Papers
  • Published:
Ecotoxicology Aims and scope Submit manuscript

A study was made of the effects of one selected acetanilide herbicide, alachlor, at concentrations of 2.0–10.0 kg ha−1 on bacterial populations, fungi, dinitrogen fixation bacteria, denitrifying bacteria, nitrifying bacteria, nitrogenase activity, acid and alkaline phosphatases, arylsulfatase and deshydrogenase. The presence of 2.0–10.0 kg ha−1 of alachlor in the soil increased the total number of bacteria and fungi. The population of denitrifying bacteria increased significantly at concentrations of 5.0–10.0 kg ha−1. However, aerobic dinitrogen fixing bacteria and nitrogenase activity decreased at alachlor concentrations of 3.5–10.0 kg ha−1. Acid and alkaline phosphatases, arylsulfatase and dehydrogenase activity decreased significantly initially at concentrations of 5.0–10.0 kg ha−1, but recovered to levels similar to those in the control. Nitrifying bacteria were not affected as a consequence of the addition of the herbicide to agricultural soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bremner, J.M. (1965) Inorganic forms of nitrogen. Agronomy 9, 1179–237.

    Google Scholar 

  • Ferrer, M.R., Gonzalez-Lopez, J. and Ramos-Cormenzana, A. (1986) Effect on some herbicides on the biological activity of Azotobacter vinelandii. Soil Biol. Biochem. 18, 237–8.

    Google Scholar 

  • Haahtela, K., Wartiovaava, T., Sundman, V. and Skujins, J. (1981) Root-associated N2 fixation (acetylene reduction) by Enterobacteriaceae and Azospirillum strains in cold-climate spodosols. Appl. Environ. Microbiol. 41, 203–6.

    Google Scholar 

  • Hardy, R.W., Holsten, R.D., Jackson, E.K. and Burns, R.C. (1968) The acetylene-ethylene assay for N2 fixation: Laboratory and field evaluation. Plant Physiol. 43, 1185–207.

    Google Scholar 

  • Lin, S.Y., Zhang, R. and Bollag, J.M. (1988) Biodegradation of metolachlor in a soil perfusion experiment. Biol. Fertility Soil 5, 276–81.

    Google Scholar 

  • Martinez-Toledo, M.V. and Gonzalez-Lopez, J. (1989) Effect of metolachlor on Azotobacter nitrogen fixation in soil. Environ. Toxicol. Chem. 8, 789–92.

    Google Scholar 

  • Martinez-Toledo, M.V., Gonzalez-Lopez, J., de laRubia, T., Moreno, J. and Ramos-Cormenzana, A. (1988) Effect of inoculation with Azotobacter chroococcum on nitrogenase activity of Zea mays roots grown in agricultural soils under aseptic and non-sterile conditions. Biol. Fertility Soils 6, 170–3.

    Google Scholar 

  • Olsen, S.R. and Dean, L.A. (1965) Phosphorus. Agronomy 9, 1035–49.

    Google Scholar 

  • Pratt, P.F. (1954) Potassium release from exchangeable and non exchangeable forms in Ohio soils. Ohio Agric. Exp. Station Res. Bull. 47.

  • Rodina, A.G. (1972) Methods in Aquatic Microbiology. Baltimore: University Park Press.

    Google Scholar 

  • Salmeron, V., Martinez-Toledo, M.V. and Gonzalez-Lopez, J. (1991) Effects of alachlor and metolachlor on the biological activity of Azospirillum brasilense grown in chemically defined and dialysed-soil media. Environ. Toxicol. Chem. 10, 493–9.

    Google Scholar 

  • Saxena, A.R., Zhang, R. and Bollag, J.M. (1987) Microorganisms capable of metabolizing the herbicide metolachlor. Appl. Environ. Microbiol. 53, 390–6.

    Google Scholar 

  • Soil Conservation Service (1975) Soil Taxonomy. A Basic System of Soil Classification for Making and Interpreting Soil Surveys. Washington, DC: US Department of Agriculture.

    Google Scholar 

  • Tabatabai, M.A. (1982) Soil enzymes. In Page, A.L., Miller, R.H. and Keeney, D.R. eds, Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties pp. 902–947. Madison, WI: American Society of Agronomy, Inc., and Soil Science Society of America, Inc.

    Google Scholar 

  • Yeomans, J.C. and Bremner, J.M. (1985) Denitrification in soil: Effect of herbicides. Soil Biol. Biochem. 17, 447–52.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pozo, C., Salmeron, V., Rodelas, B. et al. Effects of the herbicide alachlor on soil microbial activities. Ecotoxicology 3, 4–10 (1994). https://doi.org/10.1007/BF00121384

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00121384

Keywords

Navigation