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Denervated chicken breast muscle displays discoordinate regulation and differential patterns of expression of αf and β tropomyosin genes

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Summary

The expression of the α fast (αf) and β tropomyosin (TM) genes has been analysed with muscle-specific and common cDNA probes after unilateral nerve section of the pectoralis major muscle (PM) in 4-week-old chickens. The following were observed in denervated muscles. (1) The βTM mRNA, which was repressed during development, reaccumulates in a biphasic curve with the increase in the βTM protein lagging behind the changes in its mRNA. Accordingly, no βTM is seen in products translated in vitro from total and polyA+ RNA obtained 1 week after denervation. No such translation block is seen with RNA obtained from control or muscles denervated for 6 weeks. (2) No changes in the αfTM mRNA and corresponding protein are observed. (3) RNA processing of the two genes is not changed. (4) In the contralateral muscles, transitory increases in αf and βTM mRNAs are observed while the corresponding proteins remain unchanged. Our data suggest that muscle fibres display early and long-term responses to the loss of neural input which might result from a combination of changes produced by regenerative processes and reprogramming of existing fibres. Moreover, in contrast to normal development, no reciprocal changes of αf and βTM expression are seen in denervated muscles.

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Authors and Affiliations

  1. Department of Medicine, University of Chicago, Box 360, 60637, Chicago, IL, USA

    Mahesh P. Gupta, Rudolf J. Wiesner & Radovan Zak

  2. II. Physiologisches Institut, Universitat Heidelberg, Im Neuenheimer Feld 326, 6900, Heidelberg 1, Germany

    Rudolf J. Wiesner

  3. UER Biomédicale des Sts-Pères, 45 rue des Sts-Pères, 75006, Paris, France

    Vincent Mouly

  4. Unité Biochimie, Institut Pasteur, 25-28 rue du Dr Roux, 75724, Paris Cedex 15, France

    Marguerite Lemonnier

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  1. Mahesh P. Gupta
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  2. Rudolf J. Wiesner
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  3. Vincent Mouly
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  4. Radovan Zak
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  5. Marguerite Lemonnier
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Gupta, M.P., Wiesner, R.J., Mouly, V. et al. Denervated chicken breast muscle displays discoordinate regulation and differential patterns of expression of αf and β tropomyosin genes. J Muscle Res Cell Motil 14, 377–384 (1993). https://doi.org/10.1007/BF00121288

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  • DOI: https://doi.org/10.1007/BF00121288

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