Clinical & Experimental Metastasis

, Volume 1, Issue 4, pp 373–380 | Cite as

TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma

  • Patrizia Nanni
  • Carla De Giovanni
  • Pier-Luigi Lollini
  • Giordano Nicoletti
  • Giorgio Prodi
Article

A metastasizing mouse cell line (TS/A), originated from a mammary adenocarcinoma which arose spontaneously in a BALB/c female retired breeder, has been established in vitro. It displayed a remarkable morphologic heterogeneity, which is evident in plastic adherent cultures (cell types ranging from epithelial-like to fibroblast-like) as well as in semi-solid agar cultures. The TS/A line exhibited the presence of specific cytoplasmic estradiol receptor, with a binding activity of 16 fmoles/mg cytosol protein. The in vivo growth pattern was as follows: (1) a s.c. inoculum of 105 cells caused a 100 per cent tumor take and kill in syngeneic animals; mean survival time was 54 + 1 days; (2) it did not show significant transplant immunogenicity in syngeneic animals; (3) it was able to give rise to both spontaneous lung metastases and artificial lung colonies; (4) it had a high capacity to grow in H-2 matched, minor histocompatibility antigen incompatible hosts (106 cells killed 100 per cent DBA/2 mice in 58 + 2 days). This line of spontaneous mammary tumor cells is proposed as a useful model for studies on the heterogeneity of the neoplastic population in relation to metastatic spread, on tumor immunogenicity, and on therapy of mammary neoplasia.

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References

  1. [1]
    Alexander, P., 1982, The role of the host in metastasis. Accomplishments in Cancer Research 1981, edited by J. G. Fortner and J. E. Rhoads (Philadelphia-Toronto: J. B. Lippincott Company), pp. 138–148.Google Scholar
  2. [2]
    Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248–254.Google Scholar
  3. [3]
    Chandradasa, K. D., and Blears, J., 1982, Studies with a spontaneous murine tumour—I. Indetectability of host immune resistance with tumour-activated elaboration of T cell stimulatory lymphokines in vitro. European Journal of Cancer and Clinical Oncology, 18, 853–859.Google Scholar
  4. [4]
    Chen, T. R., 1977, In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research, 104, 255–262.Google Scholar
  5. [5]
    Danielson, K. G., Anderson, L. W., and Hosick, H. L., 1980, Selection and characterization in culture of mammary tumor cells with distinctive growth properties in vivo. Cancer Research, 40, 1812–1819.Google Scholar
  6. [6]
    Dexter, D. L., Kowalski, H. M., Blazar, B. A., Fligiel, Z., Vogel, R., and Heppner, G., 1978, Heterogeneity of tumor cells from a single mouse mammary tumor. Cancer Research, 38, 3174–3181.Google Scholar
  7. [7]
    Fidler, I. J., and Kripke, M. L., 1977, Metastasis results from preexisting variant cells within a malignant tumor. Science, 197, 893–895.Google Scholar
  8. [8]
    Forni, G., Varesio, L., Giovarelli, M., and Landolfo, S., 1979, Source of tumor cells influences their immune recognition by H-2-matched mice. Transplantation, 27, 433–435.Google Scholar
  9. [9]
    Fumero, S., Berruto, G. P., Pelizzola, D., Grilli, S., Buttazzi, C., Di Fronzo, G., Ronchi, E., Bozzetti, C., Mort, P., Concolino, G., Marocchi, A., Robustelli, Della Cuna, G., Zibera, C., Cerrutti, G., Ros, A., and Piffanelli, A., 1981, Results of the Italian Interlaboratory quality control program for estradiol receptor assay. Tumori, 67, 301–306.Google Scholar
  10. [10]
    Galili, N., Naor, B., Åsiö, B., and Klein, G., 1976, Induction of immune responsiveness in a genetically low-responsive tumor-host combination by chemical modification of the immunogen. European Journal of Immunology, 6, 473–476.Google Scholar
  11. [11]
    Hewitt, H. B., Blake, E. R., and Walder, A. S., 1976, A critique of the evidence for host defense against cancer based on personal studies of 27 murine tumours of spontaneous origin. British Journal of Cancer, 33, 241–259.Google Scholar
  12. [12]
    Hewitt, H. B., 1982, Animal tumor models and their relevance to human tumor immunology. Journal of Biological Response Modifiers, 1, 107–119.Google Scholar
  13. [13]
    Nanni, P., De Giovanni, C., Galli, M. C., Grilli, S., Lollini, P.-L., Nicoletti, G., and Prodi, G., 1981, Minor histocompatibility antigens do not enhance BALB/c anti-SV40 TASA response. British Journal of Cancer, 44, 588–591.Google Scholar
  14. [14]
    Wexler, H., 1966, Accurate identification of experimental pulmonary metastases. Journal of National Cancer Institute, 36, 641–645.Google Scholar

Copyright information

© Taylor & Francis Ltd 1983

Authors and Affiliations

  • Patrizia Nanni
    • 1
  • Carla De Giovanni
    • 1
  • Pier-Luigi Lollini
    • 1
  • Giordano Nicoletti
    • 1
  • Giorgio Prodi
    • 1
  1. 1.Istituto di CancerologiaUniversità di BolognaBolognaItaly

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