The effect of the bead diameter on the accuracy of two techniques used in bone ingrowth quantification, microradiography and backscattered electron imaging-scanning electron microscopy (BEI-SEM), was assessed using porous-coated implants. Two groups of seven titanium porous implants (group A: bead size 250–350 μm and group B: 500–700 μm) were implanted for 12 weeks in a canine model. After euthanasia, the same histological slides were prepared for microradiography and BEI-SEM. The percentage of bone, bone ingrowth, bone ongrowth, porosity and bone index were determined by a point counting method using images from both techniques. ANOVA and Tukey's test were used to compare the results from the different bead sizes and techniques. The results showed significant higher bone ingrowth in microradiography groups, and significant lower porosity in only the fine-bead microradiography group (group A size). Microradiography also obtained significantly higher bone ongrowth, but only for the coarse bead size group (group B). From these results it was concluded that microradiography decreases the porosity of the porous coating compared with BEI-SEM. This effect seems to be dependent on the bead diameter. The smaller the diameter, the greater the effect. Furthermore, microradiography increases bone ingrowth which seems to be affected independently of the bead diameter, becoming the most sensitive parameter to increase.
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J. O. GALANTE, W. ROSTOKER, R. LUECK and R. D. RAY, J. Bone Joint Surg. A 53 (1971) 61.
R. M. PILLIAR, H. U. CAMERON and I. MACNAB, J. Biomed. Engng. 10 (1975) 126.
R. D. BLOEBAUM, S. A. REID and K. N. BACHUS, in Proceedings of 35th Annual Meeting of the Orthopedic Research Society (ORS, Las Vegas, 1989) p. 399.
D. R. SUMNER, J. M. BRYAN, R. M. URBAN and J. R. KUSZAK, J. Orthop. Res. 8 (1990) 448.
R. E. HOLMES, H. K. HAGLER and C. A. COLETTA, J. Biomed. Mater. Res. 21 (1987) 731.
J. JOWSEY, P. J. KELLY, L.B. RIGGS, A. J. BIANCO, D. A. SCHOLZ and J. GERSHON-COHEN, J. Bone Joint Surg. A. 47 (1965) 785.
D. B. KIMMEL and S. S. J. WEBSTER, in “Bone histomorphometry techniques and interpreation”, edited by R. R. RECKER (CRC Press, Boca Raton, 1983), p. 89.
M. JASTY, C. R. BRAGDON, S. SCHUTZER, H. RUBASH, T. HAIRE and W. H. HARRIS. Scan. Microscopy, 3 (1989) 1051.
A. M. PARFITT, M. K. DREZNER, F. H. GLORIEUX, J. A. KANIS, H. MALLUCHE, P. J. MEUNIER, S. M. OTT and R. R. RECKER, J. Bone Min. Res. 2 (1987) 595.
K. N. BACHUS, A. A. HOFMANN and L. A. DAUTERMAN, in Proceedings of 34th Annual Meeting of the Orthopedic Research Society (ORS, Atlanta, 1988), p. 308.
T. ALBREKTSSON, P. I. BRANEMARK, H. A. HANSSON and J. LINDSTROM, Acta Orthop. Scand. 52 (1981) 155.
A. H. HOLMES, in “Petrographic methods and calculations” (Murby & Co., London, 1927).
R. T. DEHOFF and F. N. RHINES, in “Quantitative microscopy” (McGraw-Hill, New York, 1968).
E. E. UNDERWOOD, in “Quantitative stereology” (Addison-Wesley, Reading, MA, 1970).
E. R. WEIBEL, in “Stereological methods” (Academic Press, San Diego, 1979), p. 139.
W. J. WHITHEHOUSE, J. Microcopy 107 (1976) 183.
A. MORONI, V. CAJA, E. EGGER, F. GOTTSAUNERWOLF, L. TRINCHESE, G. ROLLO and E. Y. CHAO, in “Bioceramics. Materials, properties, applications” edited by A. RAVAGLIOLI and A. KRAJEWSKY (Chapman & Hall, New York, 1991), p. 141.
R. D. BLOEBAUM, T. A. GRUEN, P. CAMPBELL and L. D. DORR, in Proceedings of 34th Annual Meeting of the Orthopedic Research Society (ORS, Atlanta, 1988) p. 366.
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Caja, V.L., Moroni, A., Egger, E.L. et al. The effect of bead diameter on the accuracy of two current techniques used to quantify bone ingrowth in porous-coated implants. J Mater Sci: Mater Med 5, 29–32 (1994). https://doi.org/10.1007/BF00121150
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DOI: https://doi.org/10.1007/BF00121150