In vitro and in vivo investigation of a range of phosphate glass-reinforced polyhydroxybutyrate-based degradable composites

A completely degradable melt-processed composite was produced using a phosphate-based glass in the soda-lime-phosphorus pentoxide ternary phase system. In vitro degradation studies showed that the mass loss and mechanical property change could be closely correlated with the solubility rate of the reinforcing glass. The in vivo studies showed a slight inflammatory reaction, but good compatibility. With time, this inflammatory reaction disappeared and the initial reaction was interpreted as being due to a high solubility rate of the glass. The high solubility of the glass was derived not from the composition, but because the glass was in particulate form and therefore had a high surface area. This was of interest, as it showed that the polymer was highly permeable and did not encapsulate the glass as expected with a melt-processing method.

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  1. 1.

    E. FUKADA and Y. ANDO, Int. J. Biol. Macromol. 8 (1986) 361.

    Google Scholar 

  2. 2.

    S. J. HOLLAND, M. YASIN and B. J. TIGHE, Biomaterials 11 (1990) 206.

    Google Scholar 

  3. 3.

    Y. DOI, Y. KANESAWA, M. KUNIOKA and T. SAITO, Macromolecules 23 (1990) 26.

    Google Scholar 

  4. 4.

    J. C. KNOWLES, F. A. MAHMUD and G. W. HASTINGS, Clin. Mater. 8 (1991) 155.

    Google Scholar 

  5. 5.

    J. C. KNOWLES and G. W. HASTINGS, Biomaterials 12 (1991) 210.

    Google Scholar 

  6. 6.

    N. D. MILLER and D. F. WILLIAMS, Biomaterials 8 (1987) 129.

    Google Scholar 

  7. 7.

    K. E. TANNER, C. DOYLE and W. BONFIELD, in “Clinical Implant Materials”, edited by G. Heimke, U. Soltesz and A. J. C. Lee (Elsevier Science, Amsterdam, 1990).

    Google Scholar 

  8. 8.

    J. BURNIE, PhD thesis, University of Strathclyde (1982).

  9. 9.

    J. AUTIAN, Artif. Organs 1 (1977).

  10. 10.

    S. VAINIONPAA, J. KILPIKARI, J. LAIHO, P. HELEVIRTA, P. ROKKANEN and P. TORMALA, Biomaterials 8 (1987) 46.

    Google Scholar 

  11. 11.

    J. W. LEENSLAG, A. J. PENNINGS, R. R. M. BOS, F. R. ROZEMA and G. BOERING, Biomaterials 8 (1987) 311.

    Google Scholar 

  12. 12.

    J. VASENIUS, S. VAINIONPAA, K. VIHTONEN, A. MAKELA, M. MERO, P. ROKKANEN and P. TORMALA, Biomaterials 11 (1989) 501.

    Google Scholar 

  13. 13.

    P. TORMALA, J. VASENIUS, S. VAINIONPAA, J. LAIHO, T. POHJONEN and P. ROKKANEN, J. Biomed. Mater. Res. 25 (1991) 1.

    Google Scholar 

  14. 14.

    R. R. M. BOS, F. R. ROZEMA, G. BOERING, A. J. NIJENHUIS, A. J. PENNINGS, A. B. VERWEY, P. NIEUWENHUIS and H. W. B. JANSEN, Biomaterials 12 (1991) 32.

    Google Scholar 

  15. 15.

    H. OONISHI, S. KUSHITANI, M. AONO, Y. UKON, M. YAMAMOTO, H. ISHIMARU and E. TSUJI, J. Bone Joint Surg. 71B (1989) 213.

    Google Scholar 

  16. 16.

    A. M. GATTI, D. ZAFFE and G. P. POLI, Biomaterials 11 (1990) 513.

    Google Scholar 

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Knowles, J.C., Hastings, G.W. In vitro and in vivo investigation of a range of phosphate glass-reinforced polyhydroxybutyrate-based degradable composites. J Mater Sci: Mater Med 4, 102–106 (1993).

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  • Polymer
  • Mass Loss
  • High Surface
  • Inflammatory Reaction
  • High Surface Area