In vitro directional migration of 10 T1/2 fibroblasts is partially inhibited by TPA but not by its non promoting analogues. Other tumor promoters, e.g., phenobarbital, saccharin, and benzoylperoxide had no measurable effect when added in concentrations known to affect in vitro two-step transformation or intercellular communication. Inhibitors of in vitro transformation do not affect migration, except for dexamethasone, which inhibited it. Hence, there is no evidence for a general correlation between tumor promoting potential and inhibition of in vitro directional migration.
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Abbreviations
- DDT:
-
1,1,1-trichloro-2,2-bis(p-cholorophenyl) ethane
- DEXA:
-
dexamethasone
- DMSO:
-
dimethylsulfoxide
- RA:
-
retinylacetate
- SD:
-
standard deviation
- SOD:
-
superoxide dismutase
- TPA:
-
12-0-tetradecanoylphorbol-13-acetate; 4-0-Me-TPA, 4-0-methyl-TPA
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Verschueren, H. The effect of tumor promoters and antagonists on in vitro directional migration of 10T1/2 mouse embryo cells. Cell Biol Toxicol 1, 145–157 (1985). https://doi.org/10.1007/BF00120161
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DOI: https://doi.org/10.1007/BF00120161