Abstract
This paper proposes a model which correlates the exciton decay kinetics observed in picosecond fluorescence studies with the primary processes of charge separation in the reaction center of photosystem II. We conclude that the experimental results from green algae and chloroplasts from higher plants are inconsistent with the concept that delayed luminescence after charge recombination should account for the long-lived (approx. 2 ns) fluorescence decay component of closed photosystem II centers. Instead, we show that the experimental data are in agreement with a model in which the long-lived fluorescence is also prompt fluorescence. The model suggests furthermore that the rate constant of primary charge separation is regulated by the oxidation state of the quinone acceptor QA.
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Klimov, VV and Krasnovsky, AA (1982) Biophys. 27: 186–198
Klimov, VV (1984) in Adv. in Photosynthesis Research; Proc. 6th Int. Congr. Photosyn. 1: 131–138
Duysens, LNM and Sweers, H (1963) Microalgae and Photosynth Bact: 353–372
Haehnel, W, Nairn, JA, Reisberg, P, and Sauer, K (1982) Biochim. Biophys. Acta 680: 161–173
Karukstis, KK and Sauer, K (1983) J. Cell. Biochem. 23: 131–158
Holzwarth, AR (1985) in Encyclopedia Plant Physiology: Photosynthetic Membranes Eds: LA Staehelin and CJ Arntzen, in press, Springer, Berlin
Holzwarth, AR (1986) in Topics in Photosynthesis Ed: J Barber, in press, Elsevier, Amsterdam
Haehnel, W, Holzwarth, AR, and Wendler, J (1983) Photochem. Photobiol. 37: 435–443
Karukstis, KK and Sauer, K (1983) Biochim. Biophys. Acta 725: 246–253
Karukstis, KK and Sauer, K (1983) Biochim. Biophys. Acta 722: 364–371
Holzwarth, AR, Wendler, J, and Haehnel, W (1985) Biochim. Biophys. Acta 807: 155–167
Kaminski, J (1985) Acta Phys. Pol. A 67: 679–700
Nairn, JA, Haehnel, W, Reisberg, P, and Sauer, K (1982) Biochim. Biophys. Acta 682: 420–429
Butler, WL, Magde, D, and Berens, SJ (1983) Proc. Natl. Acad. Sci. USA 80: 7510–7514
Berens, SJ, Scheele, J, Butler, WL, and Magde, D (1985) Photochem. Photobiol. 42: 59–68
Gulotty, RJ, Mets, L, Alberte, RS, and Fleming, GR (1985) Photochem. Photobiol. 41: 487–496
Pearlstein,RM (1982) in Photosynthesis 1: Energy Conversion by Plants and Bacteria :293–331
Kaplanova, M and Parma, L (1984) Gen. Physiol. Biophys. 3: 127–134
Lotshaw, WT, Alberte, RS, and Fleming, GR (1982) Biochim. Biophys. Acta 682: 75–85
Shuvalov, VA, Klimov, VV, Dolan, E, Parson, WW, and Ke, B (1980) FEBS Lett. 118: 279–282
Meiburg, RF, van Gorkom, HJ, and van Dorssen, RJ (1983) Biochim. Biophys. Acta 724: 352–358
Latimer, P, Bannister, TT, and Rabinowitch, E (1956) Science 124: 585–586
Breton, J (1983) FEBS Lett. 159: 1–5
Butler, WL (1978) Ann. Rev. Plant Physiol. 29: 345–378
Rutherford,AW (1983) in The Oxygen Evolving System of Photosynth.: 63–69
Evans, MCW, Atkinson, YE, and Ford, RC (1985) Biochim. Biophys. Acta 806: 247–254
Klimov, VV, Klevanik, AV, Shuvalov, VA, and Krasnovsky, AA (1977) FEBS Lett. 82: 183–186
Diner, BA and Delosme, R (1983) Biochim. Biophys. Acta 722: 452–459
Breton, J (1982) FEBS Lett. 147: 16–20
Mauzerall, DC (1985) Biochim. Biophys. Acta 809: 11–16
Klimov, VV, Allakhverdiev, SI, and Pashchenko, VZ (1978) Dokl. Akad. Nauk. SSSR. 242: 1204–1207
Mathis,P (1984) in Adv. in Photosynthesis Research; Proc. 6. Int. Congr. Photosynth. 1: 155–158
Voznyak, VM, Sevdimaliev, RM, and Kim, VA (1984) Stud. Biophys. 99: 59–66
Klimov, VV, Ke, B, and Dolan, E (1980) FEBS Lett. 118: 123–126
Demas,JN (1983) in Excited State Lifetime Measurement.: 1–273
Borisov, AY and Il'ina, MD (1971) Biochem. USSR 36: 693–695
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Schatz, G.H., Holzwarth, A.R. Mechanisms of chlorophyll fluorescence revisited: Prompt or delayed emission from photosystem II with closed reaction centers?. Photosynth Res 10, 309–318 (1986). https://doi.org/10.1007/BF00118296
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DOI: https://doi.org/10.1007/BF00118296