Skip to main content
SpringerLink
Log in

Metastatic abilities of murine sarcomas and carcinomas. II. Relationship to cell volume and DNA index

  • Published:
Clinical & Experimental Metastasis Aims and scope Submit manuscript

The cell volume and DNA content were determined in 12 murine tumors and correlated with the ability of these tumors to metastasize spontaneously or to form lung nodules when injected i.v. The cell volume significantly correlated with spontaneous metastatic potential of investigated tumors (r = 0683; 0·02 < P < 0·05) but not with the ability of tumor cells to form artificial metastasis. The DNA index significantly correlated with both spontaneous metastasis (r=0594; 0·02<P<0·05), and lung colonization (r = 0631; 0·02< P < 0·05). The DNA index barely correlated with the cell volume (r = 0564; P =0·10).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bell, C. D., and Waizbard, E., 1986, Variability of cell size in primary and metastatic human breast carcinoma. Invasion and Metastasis, 6, 11–20.

    Google Scholar 

  2. Branda, R. F., McCormack, J. J., Prlmutter, C. A., Mathews, L. A., and Robison, S. H., 1988, Effects of folate deficiency on the metastatic potential of routine melanoma cells. Cancer Research, 48, 4529–4534.

    Google Scholar 

  3. Feichter, G. E., Goerttler, K., Haag, D., Heep, J., and Spchwechheimer, K., 1984, DNS-Messung von malignen tumoren mittels impulszytophotometrie. Deutsche Medizinische Wochenschrift, 109, 738–744.

    Google Scholar 

  4. Feichter, G. E., Mueller, A., Kaufman, M., Haag, D., Born, I. A., Abel, U., Klinga, K., Kubli, F., and Goerttler, K., 1988, Correlation of DNA flow cytometric results and other prognostic factors in primary breast cancer. International Journal of Cancer, 41, 823–828.

    Google Scholar 

  5. Frankfurt, O. S., Slocum, H. K., Rustum, Y. M., Arbuck, S. G., Pavelic, Z. P., Petrelli, N., Huben, R. P., Pontes, E. J., and Greco, W. R., 1984, Flow cytometric analysis of DNA aneuploidy in primary and metastatic human solid tumors. Cytometry, 5,71–80.

    Google Scholar 

  6. Ghani, A. M., and Krause, J. R., 1986, Investigation of cell size and nuclear clefts as prognostic parameters in chronic lymphocytic leukemia. Cancer, 58, 2233–2238.

    Google Scholar 

  7. Grdina, D. J., Hittleman, W. N., White, R. A., and Meistrich, M. L., 1977, Relevance of density, size and DNA content of tumour cells to the lung colony assay. British Journal of Cancer, 36, 659–670.

    Google Scholar 

  8. Grdina, D. J., Peters, L. J., Jones, S., and Chan, E., 1978a, Separation of cells from a murine fibrosarcoma on the basis of size. I. Relationship between cell size and age as modified by growth in vivo or in vitro. Journal of the National Cancer Institute, 61, 209–214.

    Google Scholar 

  9. Grdina, D. J., Peters, L. J., Jones, S., and Chan, E., 1978 b, Separation of cells from a murine fibrosarcoma on the basis of size. II. Differential effects of cell size and age on lung retention and colony formation in normal and preconditioned mice. Journal of the National Cancer Institute, 61, 215–220.

    Google Scholar 

  10. Jamasbi, R. J., Perkins, E., Hand, R., and Mann, R., 1985, Stability of DNA content of mouse squarnous cell carcinomas during in vitro cultivation. Proceedingeof the American Association of Cancer Research, 26, 23.

    Google Scholar 

  11. Ljungberg, B., Stenling, R., and Roos, G., 1986, DNA content and prognosis in renal cell carcinoma. Cancer, 57, 2346–2350.

    Google Scholar 

  12. Ljungberg, B., Stenling, R., and Roos, G., 1988, Tumor spread and DNA content in human renal cell carcinoma. Cancer Research, 48, 3165–3167.

    Google Scholar 

  13. McCoy, J. P., Schade, W., Merz, G. E., Esch, T., Varani, J., and Hudson, J. L., 1985, DNA content of murine fibrosarcoma cell lines with varying metastatic potential. Cancer Research, 45, 5757–5761.

    Google Scholar 

  14. Meistrich, M. L., Grdina, D. J., Meyn, R. E., and Barlogie, B., 1977, Separation of cells from mouse solid tumors by centrifugal elutriation. Cancer Research, 37, 4291–4296.

    Google Scholar 

  15. Nichi, T., Okajima, K., Fujiwara, A., Yasuda, M., Yamada, S., Taguchi, T., and Sekimoto, T., 1986, Nuclear DNA content in breast cancer, relation between ploidy patterns and lymph node metastasis. Journal of the Japanese Society for Cancer Therapy, 21, 103–107.

    Google Scholar 

  16. Oosterwijk, E., Warnaar, S. O., Zwartendijk, J., van der Velde, E. A., Fleuren, G. J., and Cornelisse, C. J., 1988, Relationship between DNA ploidy, antigen expression and survival in renal cell carcinoma. International Journal of Cancer, 42,703–708.

    Google Scholar 

  17. Pearce, V., Pathak, S., Mellard, D., Welch, D. R., and Nicolson, G. L., 1984, Chromosome and DNA analyses of rat 13762 NF mammary adenocarcinoma cell lines and clones of different metastatic potentials. Clinical and Experimental Metastasis, 2, 271–286.

    Google Scholar 

  18. Rabotti, G., 1959, Ploidy of primary and metastatic human tumours. Nature (London), 183,1276–1277.

    Google Scholar 

  19. Sato, H., and Suzuki, M., 1976, Deformability and viability of tumor cells by transcapillary passage, with reference to organ affinity of metastasis in cancer. Fundamental Aspects of Metastasis, edited by L. Weiss (Amsterdam: North-Holland Publishing Co.), pp. 311–316.

    Google Scholar 

  20. Shackney, S. E., Levine, A. M., Fisher, R. I., Nichols, P., and Lukes, R. J., 1984, The biology of tumor growth in the non-Hodgkin's lymphoma. Journal of Clinical Investigations, 73, 1201–1214.

    Google Scholar 

  21. Smith, H. S., Liotta, L. A., Hancock, M., Wolman, S., and Hackett, A., 1985, Invasiveness and ploidy of human mammary carcinomas in short term culture. Proceedings of the American Association for Cancer Research, 26, 55.

    Google Scholar 

  22. Suzuki, N., Williams, M., Hunter, N., and Withers, H. R., 1980, Malignant properties and DNA content of daughter clones from a mouse fibrosarcoma: differentiation between malignant properties. British Journal of Cancer, 42, 765–771.

    Google Scholar 

  23. Teals, D. M., Rees, R. C., Lawry, J., and Potter, C. W., 1984, Karyotypic diversity between nonmetastatic and metastatic hamster tumours of clonal origin. Treatment of Metastasis: Problems and Prospects, edited by K. Hellmann and S. A. Eccles (London: Taylor & Francis), pp. 311–314.

    Google Scholar 

  24. Tsuruo, T., Oh-hara, T., Yamori, T., Tsukagoshi, S., Ishikawa, T., and Sugano, H., 1988, Development of lymphosarcoma lines with high metastatic ability to lymph nodes and visceral organs in BALB/c mice. Clinical and Experimental Metastasis, 6, 141–152.

    Google Scholar 

  25. Volpe, J. P., Hunter, N., Basic, I., and Milas, L., 1985, Metastatic properties of murine sarcomas and carcinomas. I. Positive correlation with lung colonization and lack of correlation with s.c. tumor take. Clinical and Experimental Metastasis, 3, 281–294.

    Google Scholar 

  26. Volpe, J. P., and Milas, L., 1988, Metastatic instability of murine tumor metastases: Dependence on tumor type. Clinical and Experimental Metastasis, 6, 333–346.

    Google Scholar 

  27. Wooldridge, T. N., Grierson, H. L., Weisenburger, D., Armitage, J. O., Sanger, W. G., Collins, M. M., Pierson, J. L., Pauza, M. E., Fordyce, R., and Purtilo, D. T., 1988, Association of DNA content and proliferative activity with clinical outcome in patients with diffuse mixed cell and large cell non-Hodgkin's lymphoma. Cancer Research, 48, 6608–6613.

    Google Scholar 

  28. Zar, J. H., 1984, Biostatistical Analysis, 2nd edn (Englewood Cliffs, NJ: Prentice-Hall), pp.113–126.

    Google Scholar 

  29. Zeidman, I., 1961, The fate of circulating tumor cells. I. Passage of cells through capillaries. Cancer Research, 21, 38–39.

    Google Scholar 

Download references

Author information

Author notes

  1. I. Basic

    Present address: Department of Animal Physiology, Faculty of Natural Sciences and Mathematics, University of Zagreb, Zagreb, Yugoslavia

Authors and Affiliations

  1. Department of Experimental Radiotherapy, University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, 1515, 77030, Houston, Holcombe Boulevard, TX, U.S.A.

    J. P. G. Volpe, I. Basic & L. Milas

Authors
  1. J. P. G. Volpe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Basic
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Milas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Volpe, J.P.G., Basic, I. & Milas, L. Metastatic abilities of murine sarcomas and carcinomas. II. Relationship to cell volume and DNA index. Clin Exp Metast 8, 193–201 (1990). https://doi.org/10.1007/BF00117792

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00117792

Keywords

Search

Navigation