Abstract
The toxicity of chloride salts of physiological (zinc, manganese, nickel) and non physiological (cadmium) bivalent metal ions was studied in normal or carcinogen-transformed mouse embryo fibroblast cells. The dose response curves for toxicity to both types of cells exhibited similar shapes. The transformed cells, however, were about twice as sensitive to zinc toxicity as normal cells. When normal and transformed cells were grown together and incubated for several hours with an appropriate concentration of zinc, the malignant cells were selectively killed. Cadmium was much more toxic than the three other metal ions in both types of cells. Its toxic effect was reversed by simultaneous addition of zinc at nontoxic concentrations.
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Abbreviations
- CFA:
-
colony forming ability
- MCA:
-
3-methylcholanthrene
References
ANDRONIKASHVILI, E.L., MOSULISHVILI, L.M., BELOKOBILSKI, A.I., KHARABADZE, N.E., TEVZIEVA, T.K. and EFREMOVA, E.Y. (1974). Content of some trace elements in sarcoma M-1 DNA in dynamics of malignant growth. Cancer Res. 34:271–274.
DEAVEN, L.C. and CAMPBELL, E.W. (1980). Factors affecting the induction of chromosomal aberrations by cadmium in Chinese hamster cells. Cytogenet. Cell Genet. 26: 251–260.
DIPAOLO, J.A. and CASTO, B.C. (1979). Quantitative studies of in vitro morphological transformation of Syrian hamster cells by inorganic metal salts. Cancer Res. 39:1008–1013.
FASSETT, D.W. (1975). Cadmium: Biological effects and occurrence in the environment. Anna. Rev. Pharmacol. 15: 425–435.
FURST, A. and HARD, R.T. (1969). A survey of metal carcinogenesis. Prog. Exp. Tumor Res. 12:102–133.
GOERING, P.L. and KLAASSEN, C.D. (1984). Zinc-induced tolerance to cadmium hepatotoxicity. Toxicol. Appl. Pharmacol. 74:299–307.
GUILLE, E., GRISVARD, J. and SISSOEFF, I. (1979). Implications of reiterative DNA-metal ion complexes in the induction and development of neoplastic cells. Biol. Trace Element Res. 1:299–311.
GUNN, S.A., GOULD, T.C. and ANDERSON, W.A.D. (1963). Cadmium-induced interstitial cell tumors in rats and mice and their prevention by zinc. J. Natl. Cancer Inst. 31:745–759.
GUNN, S.A., GOULD, T.C. and ANDERSON, W.A.D. (1964) Effect of zinc on cancerogenesis. Proc. Soc. Exp. Biol. Med. 115:653–657.
HUANG, P.C., SMITH, B., BOHDAN, P. and CORRIGAN, A. (1980). Effect of zinc on cadmium influx and toxicity in cultured CHO cells. Biol. Trace Element Res. 2:211–220.
KOCH, J., WIELGUS, S., SHANKARA, B., SARYAN, L.A., SHAW, C.F. and PETERING, D. H. (1980). Zinc-, copper- and cadmium-binding protein in Ehrlich Ascites tumour cells. Biochem. J. 189:95–104.
OCHI, T. and OHSAWA, M. (1983). Induction of 6-thioguanine-resistant mutants and single-strand scission of DNA by cadmium chloride in cultured Chinese hamster cells. Mutation Res. 111:69–78.
PAPADOPOULO, D., LEVY, S., POIRIER, V., PENE, C., MARKOVITS, P. and HUBERT-HABART, M. (1981). Effects of several dimethylbenzacridines on secondary hamster embryo cells: neoplastic transformation. Eur. J. Cancer 17:179–186.
PARIZEK, J. (1960). The 3rd Oliver Bird Lecture. Sterilization of the male by cadmium salts. J. Reprod. Fert. 1:294–309.
POSWILLO, D.E. and COHEN, B. (1971). Inhibition of carcinogenesis by dietary zinc. Nature (Lond.) 231:447–448.
REZNIKOFF, C.A., BERTRAM, J.S., BRANKOW, D.W. and HEIDELBERGER, C. (1973). Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33:3239–3249.
SUNDERMAN, F.W. (1977). Metal carcinogenesis. In: Advances in Modern Toxicology. Vol. 2, Ch. 9. 257–295.
TERZAGHI, M. and LITTLE, J.B. (1976). X-radiation-induced transformation in a C3H mouse embryo derived cell line. Cancer Res. 36:1367–1374.
VALLEE, M.L. and FALCHUK, K.H. (1981). Zinc and gene expression. Phil. Trans. R. Soc. Lond. B294:185–197.
WAALKES, M.P. and POIRIER, L.A. (1984). In vitro cadmium-DNA interactions: Cooperativity of cadmium binding and competitive antagonism by calcium, magnesium and zinc. Toxicol. and Applied Pharmacol. 75:539–546.
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Frenial, JM., Coppey, J. & Little, J.B. Toxicity and mutual interactions of cadmium and zinc ions in normal and carcinogen-transformed mouse cells. Cell Biol Toxicol 2, 1–8 (1986). https://doi.org/10.1007/BF00117702
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DOI: https://doi.org/10.1007/BF00117702