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The role of biomarkers in environmental assessment (5). Invertebrate populations and communities

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Abstract

Although a number of biomarkers of pollutant exposure have been identified in invertebrate species, direct linkage with changes at population and/or community levels are poorly documented and, despite the ecological importance of invertebrates, there is no conclusive evidence that individual measurements of biochemical parameters may allow the effects of pollutants on populations and communities to be predicted. Among the various biochemical parameters used as biomarkers in invertebrates exposed to pollutants in the field, only those for which changes at population or community level can be suggested are discussed in the present review. At population and community levels, the development of resistance to pesticides and changes in behaviour, reproduction and development are analysed as putative consequences of biochemical and physiological alterations. Limits to the use of biochemical parameters as biomarkers of invertebrate exposure to pollutants are discussed. Future research trends and experimental approaches to the validation of invertebrate biomarkers in environmental pollution assessment are suggested.

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References

  • Adams, S.M., Shepard, K.K., Greeley, M.S.Jr, Jimenez, B.D., Ryan, M.G., Shugart, L.R., McCarthy, J.F. and Hinton, D.E. (1989) The use of bioindicators for assessing the effects of pollutant stress on fish.Marine Environ. Res. 28, 459–64.

    Google Scholar 

  • Anderson, R.S. (1985) Metabolism of a model environmental carcinogen by bivalve molluscs.Marine Environ. Res. 17, 137–40.

    Google Scholar 

  • Atema, J., Karnofsky, E.B. and Olesko-Szuts, S. (1979) Lobster behaviour and chemoreception: sublethal effects of number 2 fuel oil. In Jacob, F. ed.Advances in marine environmental research, pp. 122–34. Cincinnati: US Environmental Protection Agency.

    Google Scholar 

  • Atkinson, D.E. (1977)Cellular Energy Metabolism and its Regulation. New York: Academic Press.

    Google Scholar 

  • Bayne, B.L. (1989) Measuring the biological effects of pollution: the mussel watch approach.Water Sci. Technol. 21, 1089–100.

    Google Scholar 

  • Bernard, L. and Lagadic, L. (1993) Sublethal effects of dietary cyfluthrin on nutritional performance and gut hydrolase activity in larvae of the Egyptian cotton leafworm,Spodoptera littoralis.Pesticide Biochem. Physiol. 46, 171–80.

    Google Scholar 

  • Blat, A., Almar, M.M. and Romero, F.J. (1988) The effect of two sulphur-containing pesticides, fenitrothion and endosulfan, on glutathione (GSH) content and on GSHS-transferase and γ-glutamyltranspeptidase activities in midgut gland of the American red crayfishProcambarus clarkii.Drug Metab. Drug Interact. 6, 383–94.

    Google Scholar 

  • Boryslawskyj, M., Garrood, A.C., Pearson, J.T. and Woodhead, D. (1988) Elevation of glutathioneS-transferase activity as a stress response to organochlorine compounds in the freshwater musselSphaerium corneum.Marine Environ. Res. 24, 101–4.

    Google Scholar 

  • Brattsten, L.B. (1979) Ecological significance of mixed-function oxidations.Drug Metab. Rev. 10, 35–58.

    Google Scholar 

  • Brattsten, L.B. (1988) Potential role of plant allelochemicals in the development of insecticide resistance. In Barbosa, P. and Letourneau, D.K. eds.Novel aspects of insect-plant interactions, pp. 313–48. New York: John Wiley.

    Google Scholar 

  • Brattsten, L.B., Wilkinson, C.F. and Eisner, T. (1977) Herbivore-plant interactions: mixed-function oxidases and secondary plant substances.Science 196, 1349–52.

    Google Scholar 

  • Brown, T.M. and Brogdon, W.G. (1987) Improved detection of insecticide resistance through conventional and molecular techniques.Ann. Rev. Entomol. 32, 145–62.

    Google Scholar 

  • Burris, J.A., Bamford, M.S. and Stewart, A.J. (1990) Behavioral responses of marked snails as indicators of water quality.Environ. Toxicol. Chem. 9, 69–76.

    Google Scholar 

  • Capaldo, P.S. (1987) Effects of carbaryl (SEVIN) on the stage I zoeae of the red-jointed fiddler crab,Uca minax (Le Conte).Estuaries 10, 132–5.

    Google Scholar 

  • Clark, A.G. (1989) The comparative enzymology of the glutathioneS-transferases from nonvertebrate organisms.Comp. Biochem. Physiol. B 92, 419–46.

    Google Scholar 

  • Corta, H.H. (1976) Response ofGammarus pulex (L.) to modified environment. II. Reactions to abnormal hydrogen ion concentration.Crustaceana 3, 1–10.

    Google Scholar 

  • Davenport, J. (1977) A study of the effects of copper applied continuously and discontinuously to specimens ofMytilus edulis (L.) exposed to steady and fluctuating salinity levels.J. Marine Biol. Ass. UK 57, 63–74.

    Google Scholar 

  • Day, K. and Kaushik, N.K. (1987) Short-term exposure of zooplankton to the synthetic pyrethroid, fenvalerate, and its effects on rates of filtration and assimilation of the alga,Chlamydomonas reinhardii.Arch. Environ. Contam. Toxicol. 16, 423–32.

    Google Scholar 

  • Den, Besten, P.J., Herwin, H.J., Smaal, A.C., Zandee, D.I. and Voogt, P.A. (1990) Interference of polychlorinated biphenyls (Clophen A50) with gametogenesis in the sea star,Asterias rubens L.Aquat. Toxicol. 18, 231–46.

    Google Scholar 

  • Di, Giulio, R.T., Washburn, P.C., Wenning, R.J., Winston, G.W. and Jewell, C.S. (1989) Biochemical responses in aquatic animals: a review of determinants of oxidative stress.Environ. Toxicol. Chem. 8, 1103–23.

    Google Scholar 

  • Dixon, D.R. (1983) Methods for assessing the effects of chemicals on reproductive function in marine molluscs. In Vouk, V.B. and Sheehan, P.J. eds.Methods for assessing the effects of chemicals on reproductive functions, pp. 439–57. Chichester, UK: John Wiley.

    Google Scholar 

  • Donkin, P. and Widdows, J. (1986) Scope for growth as a measurement of environmental pollution and its interpretation using structure-activity relationships.Chem. Ind. 21, 732–7.

    Google Scholar 

  • Flickinger, A.L., Bruins, R.J.F., Winner, R.W. and Skilling, J.H. (1982) Filtration and phototactic behavior as indexes of chronic copper stress inDaphnia magna Straus.Arch. Environ. Contam. Toxicol. 11, 457–63.

    Google Scholar 

  • Fossi, M.C. and Leonzio, C. (eds) (1994)Nondestructive Biomarkers in Vertebrates. Boca Raton, FL: Lewis Publishers.

    Google Scholar 

  • Galgani, F., Bocquéné, G., Truquet, Ph., Chiffoleau, J.-F. and Claisse, D. (1992) Monitoring of pollutant biochemical effects on marine organisms of the French coasts.Oceanologica Acta 15, 355–64.

    Google Scholar 

  • Gardner, G.R., Yevich, P.P., Harhbarger, J.C. and Malcolm, A.R. (1991) Carcinogenicity of Black Rock Harbor sediment to the eastern oyster and trophic transfer of Black Rock Harbor carcinogens from the blue mussel to the winter flounder.Environ. Health Perspect. 90, 53–66.

    Google Scholar 

  • Georghiou, G.P. (1990) Overview of insecticide resistance. In Green, M.B., LeBaron, H.M. and Moberg, W.K. eds.Managing resistance to agrochemicals. From fundamental research to practical strategies, pp. 18–41. Washington, DC: ACS Symposium Series No. 421, American Chemical Society.

  • Gerig, V.L. (1981) Bienerigiftigkeit der Synthetisches Pyrethrine (2 Teil).Schweiz. Bienen,-Z. 104, 155–74.

    Google Scholar 

  • Giesy, J.P. and Graney, R.L. (1989) Recent developments in and intercomparisons of acute and chronic bioassays and bioindicators.Hydrobiologia 188/189, 21–60.

    Google Scholar 

  • Gould, J. (1975) Honeybee recruitment: the dance-language controversy.Science 189, 685–93.

    Google Scholar 

  • Haya, K. and Waiwood, B.A. (1983) Adenylate energy charge and ATPase activity: potential biochemical indicators of sublethal effects caused by pollutants in aquatic organisms. In Nriagu, J.O. ed.Aquatic toxicology, pp. 307–33. New York: John Wiley.

    Google Scholar 

  • Haynes, K.F. (1988) Sublethal effects of neurotoxic insecticides on insect behaviour.Ann. Rev. Entomol. 33, 149–68.

    Google Scholar 

  • Haynes, K.F. and Baker, T.C. (1985) Sublethal effects of permethrin on the chemical communication system of the pink bollworm moth,Pectinophora gossypiella.Arch. Insect Biochem. Physiol. 2, 283–93.

    Google Scholar 

  • Hill, I.R. (1985) Effects on non-target organisms in terrestrial and aquatic environments. In Leahey, J.P. ed.The pyrethroid insecticides, pp. 151–262. London, UK: Taylor and Francis.

    Google Scholar 

  • Hirata, M. and Sogawa, K. (1976) Antifeeding activity of chlordimeform for plant-sucking insects.Appl. Entomol. Zool. 11, 94–9.

    Google Scholar 

  • Hodgson, E. (1985) Microsomal mono-oxygenases. In Kerkut, G.A. and Gilbert, L.I. eds.Comprehensive insect physiology, biochemistry and pharmacology, Vol. 11, pp. 225–321. Oxford: Pergamon Press.

    Google Scholar 

  • Hopkin, S.P. (1993)In situ biological monitoring of pollution in terrestrial and aquatic ecosystems. In Calow, P. ed.Handbook of ecotoxicology, Vol. 1, pp. 397–427. Oxford: Blackwell Scientific Publications.

    Google Scholar 

  • Hoy, M.A. (1990) Pesticide resistance in arthropods natural enemies: variability and selection responses. In Roush, R.T. and Tabashnik, B.E. eds.Pesticide resistance in arthropods, pp. 203–36. New York and London: Chapman & Hall.

    Google Scholar 

  • Huggett, R.J., Kimerle, R.A., Mehrle, P.M., Jr and Bergam, H.L. (eds) (1992)Biomarkers. Biochemical, physiological, and histological markers of anthropogenic stress. Boca Raton, FL: SETAC Special Publications Series, Lewis Publishers.

  • Ivanovici, A.M. (1980) Application of adenylate energy charge to problems of environmental impact assessment in aquatic organisms.Hegol. Meeresunters. 33, 556–65.

    Google Scholar 

  • Janssen, C.R., Ferrando Rodrigo, M.D. and Persoone, G. (1993) Ecotoxicological studies with the freshwater rotiferBrachionus calyciflorus. I. Conceptual framework and applications.Hydrobiologia 255/256, 21–32.

    Google Scholar 

  • Janssens de Bisthoven, L.G., Timmermans, K.R. and Ollevier, F. (1992) The concentration of cadmium, lead, copper and zinc inChironomus gr.thummi larvae (Diptera, Chironomidae) with deformedversus normal menta.Hydrobiologia 239, 141–9.

    Google Scholar 

  • Jones, M., Folt, C. and Guarda, S. (1991) Characterizing individual, population and community effects of sublethal levels of aquatic toxicants: an experimental case study usingDaphnia.Freshwater Biol. 26, 35–44.

    Google Scholar 

  • Krebs, C.T. and Burns, K.A. (1977) Long-term effects of an oil spill on populations of the saltmarsh crabUca pugnax.Science 197, 484–7.

    Google Scholar 

  • Krieger, R.I., Feeny, P.P. and Wilkinson, C.F. (1971) Detoxication enzymes in the guts of caterpillars: an evolutionary answer to plant defenses?Science 172, 579–81.

    Google Scholar 

  • Lagadic, L. (1994) Some characteristics of digestive α-glycosidases fromBruchus affinis adults, in relation with intestinal pH.Comp. Biochem. Physiol. in press.

  • Lagadic, L. and Chararas, C. (1988) Etude des activités osidasiques digestives d'adultes deBruchus affinis en cours d'hivernation an élevage artificiel et en conditions semi-naturelles.Entomol. Exp. Appl. 48, 247–55.

    Google Scholar 

  • Landa, V., Bennettová, B., Gelbic, I., Matolín, S. and Soldán, T. (1983) Methods for the assessment of the effects of chemicals on the reproductive function of insects. In Vouk, V.B. and Sheehan, P.J. eds.Methods for assessing the effects of chemicals on reproductive functions, pp. 415–38. Chichester UK: John Wiley.

    Google Scholar 

  • Livingstone, D.R. (1991) Organic xenobiotic metabolism in marine invertebrate. In Gilles, R. ed.Advances in comparative and environmental physiology, Vol. 7, pp. 45–185. Berlin: Springer-Verlag.

    Google Scholar 

  • Livingstone, D.R. (1993) Biotechnology and pollution monitoring: use of molecular biomarkers in the aquatic environment.J. Chem. Technol. Biotechnol. 57, 195–211.

    Google Scholar 

  • Livingstone, D.R., Kirchin, M.A. and Wiseman, A. (1989) Cytochrome P-450 and oxidative metabolism in molluscs.Xenobiotica 19, 1041–62.

    Google Scholar 

  • Loya, Y. (1975) Possible effects of water pollution on the community structure of Red Sea corals.Marine Biol. 29, 177–85.

    Google Scholar 

  • Loya, Y. and Rinkevich, B. (1980) Effects of oil pollution on coral reef communities.Marine Ecol. Prog. Ser. 3, 167–80.

    Google Scholar 

  • McCarthy, J.F. and Shugart, L.R. (eds) (1990)Biomarkers of Environmental Contamination. Boca Raton, FL: Lewis Publishers.

    Google Scholar 

  • McGreer, E.R. (1979) Sublethal effects of heavy metal contaminated sediments on the bivalveMacoma balthica (L.).Marine Pollut. Bull. 10, 259–62.

    Google Scholar 

  • MacKenzie, K.E. and Winston, M.L. (1989) Effects of sublethal exposure to diazinon on longevity and temporal division of labor in the honey bee (Hymenoptera: Apidae).J. Econ. Entomol. 82, 75–82.

    Google Scholar 

  • Malins, D.C. and Ostrander, G.K. (1991) Perspectives in aquatic toxicology.Ann. Rev. Pharmacol. Toxicol. 31, 371–99.

    Google Scholar 

  • Mayer, F.L., Versteeg, D.J., McKee, M.J., Folmar, L.C., Graney, R.L., McCume, D.C. and Rattner, B.A. (1992) Physiological and nonspecific biomarkers. In Huggett, R.J., Kimerle, R.A., Mehrle, P.M., Jr and Bergman, H.L. eds.Biomarkers. Biochemical, physiological and histological markers of anthropogenic stress, pp. 5–85. Boca Raton, FL: SETAC Special Publication Series, Lewis Publishers.

    Google Scholar 

  • Metcalf, R.L. (1989) Insect resistance to insecticides.Pesticide Sci. 26, 333–58.

    Google Scholar 

  • Miura, T. and Takahashi, R.M. (1987) Impact of fenoxycarb, a carbamate insect growth regulator, on some aquatic invertebrates abundant in mosquito breeding habitats.J. Am. Mosquito Control Ass. 3, 476–80.

    Google Scholar 

  • Moreteau, B. and Ramade, F. (1980) Effects of intoxication by lindane on thecorpora allata ofLocusta migratoria (Orthoptera).Experientia 36, 1233–5.

    Google Scholar 

  • Muirhead-Thomson, R.C. (1987)Pesticide Impact on Stream Fauna with Special Reference to Macroinvertebrates. Cambridge University Press, Cambridge, UK.

    Google Scholar 

  • Mullin, C.A. (1988) Adaptative relationships of epoxide hydrolase in herbivorous arthropods.J. Chem. Ecol. 14, 1867–88.

    Google Scholar 

  • NAS/NRC (National Academy of Sciences/National Research Council) (1989)Biologic Markers in Reproductive Toxicology. Washington, DC: National Academy Press.

    Google Scholar 

  • Nicol, J.A.C., Donahue, W.H., Wang, R.T. and Winters, K. (1977) Chemical composition and effects of water extracts of petroleum on eggs of the sand-dollar (Melitta quinquiesperforata).Marine Biol. 40, 309–16.

    Google Scholar 

  • NRC (National Research Council) (1987) Committee on Biological Markers.Environ. Health Perspect. 74, 3–9.

    Google Scholar 

  • O'Brien, P.J., Elzen, G.W. and Vinson, S.B. (1985) Toxicity of azinphosmethyl and chlordimeform to parasitoidBracon mellitor (Hymenoptera: Brachonidae): lethal and reproductive effects.Environ. Entomol. 14, 891–4.

    Google Scholar 

  • Olla, B.L., Pearson, W.H. and Studholme, A.L. (1980) Applicability of behavioural measures in environmental stress assessment. In McIntyre, A.D. and Pearce, J.B. eds.Biological Effects of Marine Pollution and the Problems of Monitoring. Rapp. P.-V. Réun. Cons. Int. Explor. Mer 179, 162–73.

  • Oppenoorth, F.J. (1985) Biochemistry and genetics of insecticide resistance. In Kerkut, G.A. and Gilbert, L.I. eds.Comprehensive insect physiology, biochemistry and pharmacology, Vol. 12, pp. 713–73. Oxford, UK: Pergamon Press.

    Google Scholar 

  • Peakall, D. (1992)Animal Biomarkers as Pollution Indicators. London: Chapman & Hall.

    Google Scholar 

  • Pimentel, D., Acquay, H., Biltonen, M., Rice, P., Silva, M., Nelson, J., Lipner, V., Giordano, S., Horowitz, A. and D'Amore, M. (1992) Environmental and economic costs of pesticide use.Bioscience 42, 750–60.

    Google Scholar 

  • Ramade, F. (1965) L'action anticholinestérasique de quelques insecticides organophosphorés chezMusca domestica.Ann. Soc. Entomol. Fr. 1, 549–66.

    Google Scholar 

  • Ramade, F. (1967) Contribution à l'étude du mode d'action de certains insecticides de synthèse, plus particulièrement du lindane, et des phénomènes de résistance à ces composés chezMusca domestica L.Ann. Inst. Nat. Agron. Paris 5, 1–268.

    Google Scholar 

  • Raymond, M., Fournier, D., Bride, J.-M., Cuany, A., Bergé, J., Magnin, M. and Pasteur, N. (1986) Identification of resistance mechanisms inCulex pipiens (Diptera: Culicidae) from Southern France: insensitive acetylcholinesterase and detoxifying oxidases.J. Econ. Entomol. 79, 1452–8.

    Google Scholar 

  • Rivière, J.-L. (1970) Les carbamates insecticides.Ann. Zool. Ecol. Anim. 2, 479–508.

    Google Scholar 

  • Roush, R.T. and Daly, J.C. (1990) The role of population genetics in resistance research and management. In Roush, R.T. and Tabashnik, B.E. eds.Pesticide resistance in arthropods, pp. 97–152. New York and London: Chapman & Hall.

    Google Scholar 

  • Roush, R.T. and McKenzie, J.A. (1987) Ecological genetics of insecticide and acaricide resistance.Ann. Rev. Entomol. 32, 361–80.

    Google Scholar 

  • Scherer, E. and McNicol, R.E. (1986) Behavioural responses of stream-dwellingAcroneuria lycorias (Ins., Plecopt.) larvae to methoxychlor and fenitrothion.Aquat. Toxicol. 8, 251–63.

    Google Scholar 

  • Sheehan, P.J. (1984) Effects on individuals and populations. In Sheehan, P.J., Miller, D.R., Butler, G.C. and Bourdeau, Ph. eds.Effects of pollutants at the ecosystem level, pp. 23–50. Chichester, UK: John Wiley.

    Google Scholar 

  • Soderlund, D.M. and Bloomquist, J.R. (1990) Molecular mechanisms of insecticide resistance. In Roush, R.T. and Tabashnik, B.E. eds.Pesticide resistance in arthropods, pp. 58–96. New York and London: Chapman & Hall.

    Google Scholar 

  • Stegeman, J.J. (1985) Benzo[a]pyrene oxidation and microsomal enzyme activity in the mussel (Mytilus edulis) and other bivalve molluscs species from the Western North Atlantic.Marine Biol. 89, 21–30.

    Google Scholar 

  • Stephen, W.P. and Schriker, B. (1970) The effect of sublethal doses of parathion. II. Site of parathion activity, and signal integration.J. Apic. Res. 9, 155–64.

    Google Scholar 

  • Veldhuizen-Tsoerkan, M.B., Holwerda, D.A., de, Bont, A.M.T., Smaal, A.C. and Zandee, D.I. (1991) A field study on stress indices in the sea mussel,Mytilus edulis: application of the ‘tress approach’ in biomonitoring.Arch. Environ. Contam. Toxicol. 21, 497–504.

    Google Scholar 

  • Versteeg, D.J., Graney, R.L. and Giesy, J.P. (1988) Field utilization of clinical measures for the assessment of xenobiotic stress in aquatic organisms. In Adams, W.J., Chapman, G.A. and Landis, W.G. eds.Aquatic toxicology and hazard assessment, pp. 289–306. Philadelphia: American Society for Testing and Materials.

    Google Scholar 

  • Viarengo, A. and Canesi, L. (1991) Mussels as biological indicators of pollution.Aquaculture 94, 225–43.

    Google Scholar 

  • Villar, D., Li, M.-H. and Schaeffer, D.J. (1993) Toxicity of organophosphorus pesticides toDugesia dorotocephala.Bull. Environ. Contam. Toxicol. 51, 80–7.

    Google Scholar 

  • Villar, D., Gonzalez, J.M., Gualda, M.J. and Schaeffer, D.J. (1994) Effects of organophosphorus insecticides onDugesia tigrina: cholinesterase activity and head regeneration.Bull. Environ. Contam. Toxicol. 52, 319–24.

    Google Scholar 

  • Ward, D.V., Howes, B.L. and Ludwig, D.F. (1976) Interactive effects of predation pressure and insecticide (temefos) toxicity on populations of the marsh fiddler crabUca pugnax.Marine Biol. 35, 119–26.

    Google Scholar 

  • Warwick, W.F. (1988) Morphological deformities in Chironomidae (Diptera) larvae as biological indicators of toxic stress. In Evans, M.S. ed.Toxic contaminants and ecosystem health: a Great Lakes focus, pp. 281–320. New York: John Wiley.

    Google Scholar 

  • Warwick, W.F. (1991) Indexing deformities in ligulae and antennae ofProcladius larvae (Diptera: Chironomidae): application to contaminant-stressed environments.Can. J. Fish. Aquat. Sci. 48, 1151–6.

    Google Scholar 

  • Weis, J.S. and Weis, P. (1987) Pollutants as developmental toxicants in aquatic organisms.Environ. Health Perspect. 71, 77–85.

    Google Scholar 

  • Wentsel, R., McIntosh, A., McCafferty, W.P., Atchinson, G. and Anderson, V. (1977) Avoidance response of midge larvae (Chironomus tentans) to sediments containing heavy metals.Hydrobiologia 52, 171–5.

    Google Scholar 

  • Widdows, J. and Donkin, P. (1991) Role of physiological energetics in ecotoxicology.Comp. Biochem. Physiol. 100C, 69–75.

    Google Scholar 

  • Woin, P. and Larsson, P. (1987) Phthalate esters reduce predation efficiency of dragonfly larvae,Odonata; Aeshna.Bull. Environ. Contam. Toxicol. 38, 220–5.

    Google Scholar 

  • Young, C.L. and Stephen, W.P. (1970) The acoustical behaviour ofAcheta domesticus L. (Orthoptera: Gryllidae) following sublethal doses of parathion, dieldrin, and sevin.Oecologia 4, 143–62.

    Google Scholar 

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  1. Laboratoire d'Ecologie et de Zoologie, URA CNRS 1492, Université Paris-Sud, Bât. 442, F-91405, Orsay Cedex, France

    Laurent Lagadic, Thierry Caquet & François Ramade

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Lagadic, L., Caquet, T. & Ramade, F. The role of biomarkers in environmental assessment (5). Invertebrate populations and communities. Ecotoxicology 3, 193–208 (1994). https://doi.org/10.1007/BF00117084

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