Abstract
The incompatibility between the wild species N. africana Merxm. and the cultivated species N. tabacum has been overcome by in vitro techniques. Underdeveloped F0 seeds, placed on MS medium with supplements, produced plants which upon reaching the stage of anthesis proved to be completely sterile. Female sterility of F1 hybrids was overcome by applying tissue culture methods. Explants of stem parenchyma were grown in vitro. In every passage investigations were made of their callus production, organogenesis and cell polyploidization. The regenerants showed a great diversity in their morphological and cytological characters. Pollination of the R1 plants (N. africana × N. tabacum) with N. tabacum produced normally seeded capsules. BC1 plants were male sterile. The male sterility of the first backcross generation was preserved in BC2 and BC3, proving its cytoplasmic origin.
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Nikova, V.M., Zagorska, N.A. Overcoming hybrid incompatibility between Nicotiana africana Merxm. and N. tabacum and development of cytoplasmically male sterile tobacco forms. Plant Cell Tiss Organ Cult 23, 71–75 (1990). https://doi.org/10.1007/BF00116092
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DOI: https://doi.org/10.1007/BF00116092