Plant Cell, Tissue and Organ Culture

, Volume 23, Issue 1, pp 67–70 | Cite as

Rapid propagation of agave by in vitro tissue culture

  • L. T. Binh
  • L. T. Muoi
  • H. T. K. Oanh
  • T. D. Thang
  • D. T. Phong
Article

Abstract

A procedure for rapid propagation of Agave (A. cantala Roxb., A. fourcroydes Lem. and A. sisalana Perrine, (Agavaceae) have been developed. The explants were excised from stolon plantlets, sterilized and cultivated on Murashige and Skoog (MS) basal medium containing 2% sucrose, 10% coconut water and 0.8% agar. The addition of following combination of growth substances—0.075 mgl-1 naphthalenacetic acid (NAA)+0.1 mgl-1 indolylbutyric acid (IBA)+0.5 mgl-1 kinetin (KIN) caused an extensive proliferation of multiple shoot primordia. Subcultures of these on the same medium were successful for the multiplication with an index of 3–4 times per 4 weeks subculture period. Shoots were rooted on hormone free MS medium and then transferred into a sand bed for acclimation before field planting.

Key words

acclimation Agave multiple shoot propagation rooting 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Groenewald EG, Koeleman A & Wessels DCJ (1975) Callus formation from seed tissue of Aloe pretoriensis Pole Evens. Z. Pflanzenphysiol. 75: 270–272Google Scholar
  2. 2.
    Groenewald EG, Wessels DCJ & Koelman A (1977) Callus formation and subsequent plant regeneration from seed tissue of an Agave species (Agavaceae). Z. Pflanzenphysiol. 81: 369–373Google Scholar
  3. 3.
    Kaul K & Sabharwal PS (1972) Morphogenetic studies on Haworthea: establishment of tissue culture and control of differentiation. Am. J. Bot. 59: 377–385Google Scholar
  4. 4.
    Kunisaki JT (1975) In vitro propagation of Cordyline terminalis (L.). Kunth. Hort. Science 10: 601–602Google Scholar
  5. 5.
    Lee JA (1977) Plant fibers. In Christie BR (Ed.) Handbook of Plant Science in Agriculture, Vol II (pp 178–179). CRC Press, Inc., Boca Raton, FloridaGoogle Scholar
  6. 6.
    Lock GW (1962) Sisal. John Wiley & Sons, New YorkGoogle Scholar
  7. 7.
    Mee GWP (1978) Propagation of Cordyline terminalis from callus culture. Hort. Science 13: 660Google Scholar
  8. 8.
    Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497Google Scholar
  9. 9.
    Powers DE & Backhaus RA (1989) In vitro propagation of Agave arizonica Gentry & Weber. Plant Cell Tiss. Org. Cult. 16: 57–60Google Scholar
  10. 10.
    Sharma OP & Khanna P (1979) Studies on steroidal sapogenins from tissue cultures of Agave wightii. J. Nat. Prod. 43: 459–462Google Scholar
  11. 11.
    Wakasa K (1979) Variation in the plants differentiated from the tissue culture of pineapple. Jpn. J. Breed. 29: 13–22Google Scholar
  12. 12.
    Wessels DJC, Groenewald EG & Koeleman A (1976) Callus formation and subsequent shoot and root development from leaf tissue of Haworthia planifolia var. cf. var. setulifera v. Poelln. Z. Pflanzenphysiol. 78: 141–145Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • L. T. Binh
    • 1
  • L. T. Muoi
    • 1
  • H. T. K. Oanh
    • 1
  • T. D. Thang
    • 1
  • D. T. Phong
    • 1
  1. 1.Institute of BiologyNCSR VietnamHanoiVietnam

Personalised recommendations