Abstract
Pigment as well as isozyme variations were observed among aspen (Populus tremuloides Michx.) plants regenerated from callus cultures. Out of more than 600 plantlets, two chimeric plants (one with green base and two albino shoots and the other with an albino shoot) were produced. Callus derived from albino shoots produced albino as well as chimeric plants when transferred to shoot inducing medium. Isozyme patterns of 119 plants were examined by starch gel electrophoresis. Thirty plants showed variation in shikimic dehydrogenase isozyme and 41 in isocitric dehydrogenase. Variation was also observed in malate dehydrogenase and phosphoglucose isomerase. No variation was seen in 6-phosphogluconate dehydrogenase. Pigment variation was not associated with any isozyme changes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzyladenine
- IBA:
-
indole-3-butyric acid
- GD:
-
Gresshoff & Doy medium
- WPM:
-
woody plant medium
- SKD:
-
shikimic dehydrogenase
- IDH:
-
isocitric dehydrogenase
- MDH:
-
malate dehydrogenase
- PGI:
-
phosphoglucose isomerase
- 6-PGD:
-
6-phosphogluconate dehydrogenase
References
Adams WT & Joly RJ (1980) Genetics of allozyme variants in loblolly pine. J. Hered. 71: 33–40
Bhaskaran S, Smith RH, Paliwal S & Schertz KF (1987) Somaclonal variation from Sorghum bicolor (L.) Moench cell culture. Plant Cell Tiss Org Cult 9: 189–196
Cheliak WM & Dancik BP (1982) Genic diversity of natural populations of a clone-forming tree Populus tremuloides. Can. J. Genet. Cytol. 24: 611–616
Chua BU, Kunisaki JT & Sagawa Y (1981) In vitro propagation of Dracaena marginata Tricolor. HortScience 16: 494
Clayton JW & Tretiak DN (1972) Amino-citrate buffers for pH control in starch gel electrophoresis. J. Fish Res. Board. Can. 29: 1169–1172
Davies PA, Pallotta MA, Ryan SA, Scowcroft WR & Larkin PJ (1986) Somaclonal variation in wheat: Genetic and cytogenetic characterisation of alcohol dehydrogenase 1 mutants. Theor. Appl. Genet. 72: 644–653
Evans DA & Sharp WR (1986) Applications of somaclonal variation. Bio/Technol. 4: 528–532
Evans DA & Sharp WR (1986) Somaclonal and gametoclonal variation. In: Evans DA, Sharp WR & Ammirato PV (Eds) Handbook of Plant Cell Culture, Vol 4 (pp 97–132). Macmillan, NY
Freytag AH, Rao-Arelli AP, Anand SC, Wrather JA & Owens LD (1989) Somaclonal variation in soybean plants regenerated from tissue culture. Plant Cell Rep. 8: 199–202
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean roots cells. Exp. Cell Res. 50: 151–158
Green KA, Zasada JC & Van Cleve K (1971) An albino aspen sucker. For. Sci. 17: 272
Gresshoff PM & Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (Tomato). Planta 107: 161–170
Heinz DJ & Mee GWP (1971) Morphologic, cytogenetic and enzymatic variation in Saccharum species hybrid clones derived from callus tissue. Am. J. Bot. 58: 257–262
Hyun JO, Rajora OP & Zsuffa L (1987) Genetic variation in trembling aspen in Ontario based on isozyme studies. Can. J. For. Res. 17: 1134–1138
Larkin PJ, Ryan SA, Brettell RIS & Scowcroft WR (1984) Heritable somaclonal variation in wheat. Theor. Appl. Genet. 67: 443–455
Latunde-Dada AO & Lucas JA (1983) Somaclonal variation and reaction of Verticillium wilt in Medicago sativa L. plants regenerated from protoplasts. Plant Sci. Lett. 32: 205–211
Lester DT & Berbee JG (1977) Within-clone variation among black poplar trees derived from callus culture. For. Sci. 23: 122–131
Lloyd G & McCown B (1980) Commercially feasible micropropagation of mountain laurel Kalmia latifolia by use of shoot tip culture. Combined Proc. Intl Plant Prop. Soc. 30: 421–427
Matern U, Strobel G & Shepard J (1978) Reaction to phytotoxins in a potato population derived from mesophyll protoplasts. Proc. Natl Acad. Sci. USA 75: 4935–4939
Meyer MM (1982) The use of flowers and leaves as explant sources for propagation of herbaceous and woody perennial plants. In Vitro 18: 293 (abstract 79)
Mitton JB, Linhart YB, Sturgeon KB & Hamrick JL (1979) Allozyme polymorphisms detected in mature needle tissue of ponderosa pine. J. Hered. 70: 86–89
Noh EW & Minocha SC (1986) High efficiency shoot regeneration from callus of quaking aspen (Populus tremuloides Michx.). Plant Cell Rep. 5: 464–467
O'Malley DM, Wheeler NC & Guries RP (1980) A manual for starch gel electrophoresis. Staff paper ser #11 Coll of Agri and Life Sci Univ of Wisconsin-Madison. pp 16
Shepard JF, Bidney D & Shahin E (1980) Potato protoplasts in crop improvement. Science 208: 17–24
Shoemaker RC, Cody AM & Palmer RG (1985) Characterization of a cytoplasmically inherited yellow foliar mutant (cyt-Y3) in soybean. Theor. Appl. Genet. 69: 279–284
Squillace AE & Kraus JF (1963) The degree of natural selfing in slash pine estimated from albino frequencies. Silvae Genet. 12: 46–50
Sunblade TM & Meyer MM (1982) In vitro culture of variegated Saintpaulia and Espiscia plants. HortScience 17: 532 (Abstract 426)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Noh, EW., Minocha, S.C. Pigment and isozyme variation in aspen shoots regenerated from callus culture. Plant Cell Tiss Organ Cult 23, 39–44 (1990). https://doi.org/10.1007/BF00116087
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00116087