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Direct analysis of root zone data in a microculture system

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Abstract

The feasibility of using a whole plant microculture system coupled with image analysis to observe and quantify elusive root growth phenomena was demonstrated. Subtle differences in root initiation and growth rate for maple microcuttings inserted into three distinct rooting media were recurrently registered over the span of the rooting phase in terms of root length, number, and weighted density (equivalent to fresh weight) without disturbing the rooting environment. This method provided a non-intrusive time-course assay for take-all disease symptom expression on wheat lines (degree and rate of root lesion development), which paralleled the relative resistance rated in field plots. Although agar-solidified media had a relatively high light transmission (nearly 84% through 6.6 cm medium depth), roots could only be clearly perceived through a thickness of about 2 cm, and were often completely obscured when embedded to a depth greater than 5 cm. However, there was no detectable loss of definition for roots observed through Gelrite-solidified media (non-diffuse light transmission of 97%) up to a depth of 6.6 cm.

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Abbreviations

BA:

benzyl adenine

FW:

fresh weight

IBA:

indole-3-butyric acid

TDZ:

thidiazuron

WD:

weighted density

References

  1. Arkin GF (1981) Root zone modification: systems considerations and constraints. In: Arkin GF & Taylor HM (Eds) Modifying the Root Environment to Reduce Crop Stress (p 393–403). Am. Soc. Agric. Eng. Monograph 4, St Joseph, Michigan

  2. Bentz SE, Stimart DP & McIntosh MS (1985) Root and shoot growth patterns of newly rooted woody plants. J. Am. Soc. Hortic. Sci. 110:308–313

    Google Scholar 

  3. Holden J (1976) Infection of wheat seminal roots by varieties of Phialophora radicola and Gaeumannomyces graminis. Soil. Biol. Biochem. 8:109–119

    Google Scholar 

  4. Ivanicka J (1987) In vitro micropropagation of Mulberry, Morus nigra L.. Sci. Hortic. 32:33–39

    Google Scholar 

  5. Lakso AN, Reisch BI, Mortensen J & Roberts MH (1986) Carbon dioxide enrichment for stimulation of growth of in vitro-propagated grapevines after transfer from culture. J. Am. Soc. Hortic. Sci. 111:634–638

    Google Scholar 

  6. Lee N, Wetzstein HY & Sommer HE (1986) The effect of agar vs. liquid medium on rooting in tissue-cultured sweetgum. HortScience 21:317–318

    Google Scholar 

  7. Meyer MJ, Smith MAL & Knight SL (1989) Salinity effects on St. Augustinegrass: A novel system to quantify stress response. J. Plant Nutr. 12:893–908

    Google Scholar 

  8. Murakami T & Yoneyama T (1988) Comparison of root length of two rice (Oryza sativa L.) varieties by using an image analyzer. Plant and Soil 105: 287–289

    Google Scholar 

  9. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497

    Google Scholar 

  10. Pearson RW (1974) Significance of rooting pattern to crop production and some problems of root research. In: Carson EW (Ed) The Plant Root and Its Environment (pp 240–270). Univ Press of Virginia, Charlottesville, VA

    Google Scholar 

  11. Penrose L (1985) Evidence for resistance in wheat cultivars grown in sand culture to the take-all pathogen, Gaeumannomyces graminis var. tritici. Ann. Appl. Biol. 107: 105–108

    Google Scholar 

  12. Pieper M & Smith MAL (1988) A model whole plant microculture system for Kentucky bluegrass (Poa pratensis L.) Crop Sci. 28:611–614

    Google Scholar 

  13. Smith MAL & Neely D (1981) Screening woody ornamental cuttings for propagation diseases. Plant Dis. 65: 893–895

    Google Scholar 

  14. Smith MAL, Spomer LA, Meyer MJ & McClelland MT (1989) Non-invasive image analysis evaluation of growth during plant micropropagation. Plant Cell, Tiss. Org. Cult. 19:91–102

    Google Scholar 

  15. Wallwork H (1989) Screening for resistance to take-all in wheat, triticale and wheat-triticale hybrid lines. Euphytica 40:103–109

    Google Scholar 

  16. White PR (1943) A Handbook of Plant Tissue Culture. The Jacques Cattell Press, Lancaster, Pennsylvania

    Google Scholar 

  17. Wulster GJ (1985) A nondestructive method for measuring root system surface area. HortScience 20:1058–1060

    Google Scholar 

  18. Young E & Werner J (1984) A nondestructive method for measuring shoot and root fresh weights. HortScience 19:554–555

    Google Scholar 

  19. Zimmerman RH (1984) Rooting apple cultivars in vitro: Interactions among light, temperature, phloroglucinol and auxin. Plant Cell, Tissue Org. Cult. 5:301–311

    Google Scholar 

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Authors and Affiliations

  1. Dept of Horticulture, Univ. of Illinois, 61801, Urbana, IL, USA

    M. A. L. Smith, L. A. Spomer & M. T. McClelland

Authors
  1. M. A. L. Smith
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  2. L. A. Spomer
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  3. M. T. McClelland
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Smith, M.A.L., Spomer, L.A. & McClelland, M.T. Direct analysis of root zone data in a microculture system. Plant Cell Tiss Organ Cult 23, 21–26 (1990). https://doi.org/10.1007/BF00116085

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  • DOI: https://doi.org/10.1007/BF00116085

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