In this paper the possibility of growing human bone marrow cells (HBMC) on dense calcium carbonate in conditions driving these cells towards osteogenesis is explored. It was observed that HBMC were able to adhere and multiply and can be driven best towards osteogenesis in the presence of Dex and 1,25(OH)D3 (as regards ALP activity). Specific morphological features of cells were observed by scanning electron microscopy when cells were cultured on this material. Further experiments are needed to explain these characteristics.
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G. GUILLEMIN, J. L. PATAT, J. FOURNIE and M. CHETAIL, J. Biomed. Mater. Res. 21 (1987) 557.
G. GUILLEMIN, A. MEUNIER, P. DALLANT, P. CHRISTEL, J. C. POULIQUEN and L. SEDEL, J. Biomed. Mater. Res. 23 (1989) 765.
M. OWEN, in “Bone and mineral research/3”, Edited by W. A. Peck (Elsevier Science, Amsterdam 1985) p. 1.
J. T. Triffitt, Acta Orthop. Scand. 58 (1987) 673.
J. N. Beresford, Clin. Orthop. Related Res. 240 (1989) 270.
J. E. Davies, R. Chernecky, B. Lowenberg and A. Shiga, Cells and Materials 1 (1991) 3.
C. A. G. McCulloch and H. C. Tenenbaum, Anat. Rec. 215 (1986) 397. 6. C.
C. Maniatopoulos, J. Sodek and A. H. Melcher, Cell Tissue Res. 254 (1988) 317.
P. S. Leboy, J. N. Beresford, C. Delvin and M. Owen, J. Cellular Physiol. 146 (1991) 370.
D. C. West, A. Sattar and S. Kumar, Anal. Biochem. 147 (1987) 289.
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Petite, H., Christel, P. & Triffitt, J.T. Culture of human bone marrow cells on non-porous calcium carbonate and plastic: preliminary results. J Mater Sci: Mater Med 5, 368–370 (1994). https://doi.org/10.1007/BF00058965
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DOI: https://doi.org/10.1007/BF00058965