Hemocompatibility can be conferred on a biomaterial by covering this material with a monolayer of endothelial cells. The endothelial cell is an epithelial cell of mesenchymal origin, that features a specific phenotype with homotypic intercellular interactions and with specialized cell-matrix interactions. These interactions are mandatory to the normal barrier function and the non-thrombogenicity of the endothelial monolayer and are maintained in vivo at shear stresses ranging from 10-5 to 10-3 N cm-2. The endothelial monolayer grafted on a biomaterial should meet similar requirements. We have constructed a rotating disc device to investigate the effects of differential shear stresses on cell-cell and cell-matrix interactions in a monolayer of endothelial cells grafted on a disc-shaped biomaterial. The range of shear stresses that are being applied by the device vary from 0–10-4 N cm-2 to 0–2×10-3 N cm-2. In a series of experiments with discs of plasma discharge treated polycarbonate (PC) that are coated with fibronectin, it has been shown that a monolayer of endothelial cells grafted on these discs starts to lose intercellular contacts and cell-fibronectin interactions at shear stresses of 10-4 N cm-2. Coating of the PC discs with a complex extracellular matrix, synthesized by arterial smooth muscle cells in culture, prior to endothelial cell seeding results in the formation of a monolayer, which retains its integrity at shear stresses up to 2×10-3 N cm-2.
Similar content being viewed by others
References
R. ROSENBERG and J. ROSENBERG, J. Clin. Invest. 74 (1984) 1.
E. D. HAY, J. Cell Biol. 91 (1981) 205S.
R. O. HYNES, Cell 48 (1987) 549.
E. RUOSLAHTI and M. D. PIERSCHBACHER, Science 238 (1987) 491.
S. P. MASSIA and J. A. HUBBELL, J. Biomed. Mater. Res. 25 (1991) 223.
E. A. JAFFE, R. L. NACHMAN, C. G. BECHER and C. R. MINICK, J. Clin. Invest. 52 (1973) 2745.
R. ROSS, J. Cell Biol 50 (1971) 172.
K. J. PRATT, S. K. WILLIAMS and B. E. J. JARRELL, J. Biomed. Mater. Res. 23 (1989) 1131.
J. C. F. POOLE, A. G. SANDERS and H. W. FLOREY, J. Pathol. Bacteriol. 75 (1958) 133.
E. S. REYNOLDS, J. Cell biol. 17 (1963) 208.
V. G. LEVICH, in “Physicochemical hydrodynamics” (Prentice-Hall, Englewood Cliffs, NJ, 1962) p. 60.
J. A. VAN MOURIK, O. C. LEEKSMA, J. H. REINDERS, P. DE GROOT, and J. ZANDBERGEN-SPAARGAREN, J. Biol. Chem. 260 (1985) 11300.
S. M. ALBELDA, W. A. MULLER, C. A. BUCK and P. J. NEWMAN, J. Cell Biol. 114 (1991) 1059.
C. C. HAUDENSCHILD, in “Biology of the endothelial cells”, edited by E. J. Jaffe (Nijhoff, Boston, 1984) p. 129.
C. M. REGEN and A. F. HORWITZ, J. Cell Biol. 119 (1992) 1347.
E. DEJANA, M. G. LAPUGNANI, M. GIORGI, M. GABOLI, A. B. FEDERICI, Z. M. RUGGERI and P. C. MARCHISIO, J. Cell Biol. 109 (1989) 367.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Reutelingsperger, C.P.M., Van Gool, R.G.J., Heijnen, V. et al. The rotating disc as a device to study the adhesive properties of endothelial cells under differential shear stresses. J Mater Sci: Mater Med 5, 361–367 (1994). https://doi.org/10.1007/BF00058964
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00058964