Skip to main content
SpringerLink
Log in

Evolution of digestibility by Hind III: an analysis by light and electron microscopy

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Digestion of Chinese hamster metaphase chromosomes from the Don cell line by Hind III restriction endonuclease followed by Giemsa staining were analysed by light and electron microscopy. The evolution of digestibility was studied and four digestion stages were characterized by different levels of chromosome structure. Three different condensation stages were established according to morphological criteria of length, width and separation among chromatids. It was observed that there are statistically significant differences in the digestion progress at the three condensation stages previously defined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bianchi, N. O., Bianchi, M. S. & Cleaver, J. E., 1984. The action of ultraviolet light on the patterns of banding induced by restriction endonucleases in human chromosomes. Chromosoma 90: 133–138.

    Google Scholar 

  • Comings, D. E., 1978. Mechanisms of chromosome banding and implications for chromosome structure. Ann. Rev. Genet. 12: 25–46.

    Google Scholar 

  • Goyanes, V. J. & Mendez, J., 1982. Karyotyping chromosomes by electron microscopy. II. A method for the sequential examination of spread and banded metaphases by light and electron microscopy. Hum. Genet. 62: 355–357.

    Google Scholar 

  • Lica, L. & Hamkalo, B., 1983. Preparation of centromeric heterochromatin by restriction digestion of mouse L 929 cells. Chromosoma 88: 42–49.

    Google Scholar 

  • Mezzanotte, R., Bianchi, U., Vanni, R. & Ferrucci, L., 1983. Chromatin organization and restriction endonuclease activity on human metaphase chromosomes. Cytogenet. Cell Genet. 36: 562–566.

    Google Scholar 

  • Mezzanotte, R. & Ferrucci, L., 1984. Alterations induced in mouse chromosomes by restriction endonucleases. Genetica 64: 123–128.

    Google Scholar 

  • Miller, D. A., Choi, J. C. & Miller, O. J., 1983. Chromosome localization of higher repetitive human DNAs and amplified ribosomal DNA with restriction enzymes. Science 219: 395–397.

    Google Scholar 

  • Sahasrabuddhe, C. G., Pathak, S. & Hsu, T. C., 1978. Responses of mammalian chromosomes to endonucleases digestion. Chromosoma 69: 331–337.

    Google Scholar 

  • Sumner, A. T., 1982. The nature and mechanisms of chromosome banding. Cancer Genet. Cytogenet. 61: 59–87.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Genética, Colegio Universitario de La Coruña, Universidad de Santiago, 15071, La Coruña, Spain

    J. Méndez, A. M. González & A. M. Insua

  2. Sección de Genética, Hospital ‘T. Herrera’ (INSALUD), 15006, La Coruña, Spain

    V. J. Goyanes

Authors
  1. J. Méndez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. González
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Insua
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. J. Goyanes
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Méndez, J., González, A.M., Insua, A.M. et al. Evolution of digestibility by Hind III: an analysis by light and electron microscopy. Genetica 78, 57–62 (1986). https://doi.org/10.1007/BF00058675

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00058675

Keywords

Search

Navigation