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Growth and metastatic behavior of human tumor cells implanted into nude and beige nude mice

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The growth and metastatic behavior of several human tumor lines grown in adult nude mice, nude mice pretreated with antiserum against asialo GM1 glycoprotein, and beige nude mice were studied. The cell lines were all injected s.c. and i.v. A human colon carcinoma line was also injected into the spleen, and two human renal carcinoma lines were injected into renal subcapsule. All the tumor lines grew as fast or faster in adult nude mice compared with beige nude mice. There were no discernible differences in the production of experimental lung metastasis among the three groups of mice, but human colorectal carcinoma cells and human renal carcinoma cells produced more metastases in nude mice than in beige nude mice after intrasplenic or renal subcapsule injection, respectively. In vitro cytotoxicity assays confirmed that adult nude mice had high levels of natural killer (NK) cell activity whereas nude mice pretreated with anti-asialo GM1 serum and beige nude mice did not. The in vitro NK cell activity of nude mice was demonstrable against mouse lymphoma cells but not against human leukemia cells which were sensitive to lysis by human NK cells. These results suggest that the implantation of human tumor cells into beige nude mice, which are deficient in NK cell activity does not provide an advantageous model for the study of growth and metastasis of human neoplasms.

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Abbreviations

NK:

natural killer

HCC:

human colon carcinoma

HRCC:

human renal cell carcinoma

anti-asGM1:

anti-asialo GM1

i.s.:

intrasplenic

RSC:

renal subcapsule

HBSS:

Ca2+- and Mg2+-free Hanks' balanced salt solution

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Author notes

  1. Seiji Naito

    Present address: Department of Urology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, 812, Higashi-ku, Fukuoka, Japan

  2. Raffaella Giavazzi

    Present address: Mario Negri Institute for Pharmacological Research, Via Gavazzeni, 11, 24100, Bergamo, Italy

Authors and Affiliations

  1. Department of Cell Biology-173, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute at Houston, 1515 Holcombe Boulevard, 77030, Houston, Texas, U.S.A.

    Seiji Naito, Raffaella Giavazzi, Shirley M. Walker, Kyogo Itoh & Isaiah J. Fidler

  2. Mammalian Genetics, NCI-Frederick Cancer Research Center, 210701, Frederick, Maryland, U.S.A.

    Joseph Mayo

Authors
  1. Seiji Naito
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  6. Isaiah J. Fidler
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Naito, S., Giavazzi, R., Walker, S.M. et al. Growth and metastatic behavior of human tumor cells implanted into nude and beige nude mice. Clin Exp Metast 5, 135–146 (1987). https://doi.org/10.1007/BF00058059

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  • DOI: https://doi.org/10.1007/BF00058059

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