Abstract
The distribution and the behaviour of the nucleolus organizer regions (NORs) were analysed during the spermatogenesis and oogenesis of K. flavicollis with the silver staining method. The Ag-stainability of the NORs increases in growing spermatocytes up to pachytene and is absent during the remainder of the meiotic prophase. During female meiosis the nucleolar material undergoes a more complex transformation. It is active until pachytene; in early diplotene the mass of silver stainable material progressively increases as an effect of rDNA amplification. By the end of meiotic prophase the nucleolar strands disappear and a large nucleolus is rebuilt in the mature oocyte.
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Buys, C. H. C. M. & Osinga, J., 1980. Abundance of protein-bound sulphydryl and disulfide groups at chromosomal nucleolus organizing regions. Chromosoma 77: 1–11.
Czaker, R., 1978. Silver staining in trascriptionally active NORs of meiotic and mitotic cells in Acheta domesticus L. (Orthoptera). Chromosoma 68: 187–193.
Fontana, F. & Goldoni, D., 1985. The structure of an interchange chain of four chromosomes in Reticulitermes lucifugus (Isoptera: Rhinotermitidae). Genetica 66: 89–91.
Fontana, F. & Goldoni, D., 1989. Silver staining patterns in mitotic and meiotic chromosomes of Kalotermes flavicollis Fabr. (Insecta, Kalotermitidae). Cytobios 58: 19–23.
Goodpasture, C. & Bloom, S. E., 1975. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53: 37–50.
Hofgartner, F. J., Schmid, M., Krone, W., Zenzes, M. T. & Engel, W., 1979. Pattern of activity of nucleolus organizer during spermatogenesis in mammals as analyzed by silver-staining. Chromosoma 71: 197–216.
Howell, W. M., 1977. Visualization of ribosomal gene activity. Silver stain proteins associated with rRNA transcribed from oocyte chromosomes. Chromosoma 62: 361–367.
Howell, W. M. & Black, D. A., 1980, Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.
Lima-de-Faria, A., 1983. Molecular evolution and organization of the chromosome, pp. 162–166. Elsevier, Amsterdam-New York-Oxford.
Lima-de Faria, A., Nilsson, B., Cave, D., Puga, A. & Jaworska, H., 1968. Tritium labelling and cytochemistry of extra DNA in Acheta. Chromosoma 25: 1–20.
Miller, D. A., Dev, V G., Tantravhai, R. & Miller, O. J., 1976. Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Expl. Cell Res. 101: 235–243.
Rufas, J. S., Esponda, P. & Gosalvez, J., 1985. NOR and nucleolus in the spermatogenesis of acridoid grasshoppers. Genetica 66: 139–144.
Satya-Prakash, K. L. & Pathak, S., 1984. Silver staining pattern of male meiosis in the house cricket. J. Heredity 75: 319–320.
Schmid, M., Loser, C., Schmidke, J. & Engel, W., 1982. Evolutionary conservation of a common pattern of activity of nucleolus organizer during spermatogenesis in vertebrates. Chromosoma 86: 149–179.
Suya, J. A. & Rufas, J. S., 1987. Nucleolar meiotic cycle in Orthoptera. Cell Biol. Rep 11: 289–299.
Telfer, W. H., 1975. Development and physiology of the oocytenurse cell syncytium. In: J. E. Treherne, M. J. Berridge and V. B. Wigglesworth (Ed.), Adv. Ins. Physiol., 11: 223–319. Academic Press, London.
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Fontana, F., Goldoni, D. & Bergamini, M. Patterns of activity of nucleolar organizer regions during spermatogenesis and oogenesis in Kalotermes flavicollis Fabr. (Insecta: Isoptera) analyzed by silver staining. Genetica 82, 11–15 (1990). https://doi.org/10.1007/BF00057668
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DOI: https://doi.org/10.1007/BF00057668