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Plant tissue culture of Secale: A review

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Summary

Plant tissue culture of rye employs different parts of plant bodies originating from various stages of rye ontogenesis. For the culture initiation mainly diploid and after that tetraploid forms were used, however, haploids and triploids were subject of investigation. The development of plant regeneration via somatic embryogenesis or organogenesis required the using of numerous basal media with various plant growth hormones. Haploidization appeared to be the most difficult problem and only interspecies hybridization helped to overcome this problem. Wide sexual intergenera and interspecies hybridization in cereals require the development of proembryo and immature embryo culture system. Using the ‘nurse culture’ basing on immature endosperm resulted in getting fully formed rye plants. There is no progress in somatic cell genetic manipulation of rye because of lack of plant regeneration system in mesophyll green leaf and suspension protoplast cultures.

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Abbreviations

2, 4, 5, Cl3POP...:

2, 4, 5-Cl3-phenoxypropionic acid, dicamba...-3, 6-dichloroasinic acid

GA3...:

gibberellic acid

BA...:

benzyladenine

IBA...:

3-indolebutric acid

IAA...:

indoleacetic acid

NAA...:

1-naphtylacetic acid

2, 4-D...:

2, 4-dichlorophenoxyacetic acid

2, 4, 5-T...:

2, 4, 5-trichloroacetic acid

p-CPA...:

parachlorophenoxyacetic acid

CM...:

coconut milk

CW...:

deproteined coconut milk

Kin:

Kinetin

References

  • Anonyn 77. Rye mesophyll protoplast: isolation, culture and formation. Acta Gen. Smica, 4: 242–247.

  • A min & M., Okomoto, 1976. Chromosome varia llus cells derived from Secale cereale L. with and without romosome. Jap. J. Gen. 51: 297–303.

    Google Scholar 

  • Bebeli, P., A., Karp & P.J., Kaltsikes, 1988. Plant regeneration and somaclonal variation from cultured immature embryos of sister, lines of rye and triticale differing in their content of heterochromatin. Theor. Appl. Genet. 75: 929–956.

    Google Scholar 

  • Blayeds, D.F., 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissues. Physiol. Plant. 19: 748–753.

    Google Scholar 

  • Burstrom, H., 1941. Informative effects of carbohydrate on root growth. Bot. Noits, 94: 310–334.

    Google Scholar 

  • Carew, P.D. & A.E., Schwarting, 1958. Production of rye embryo callus. Bot. Gaz., 119: 237–239.

    Google Scholar 

  • Chang, Z. & J., Hong-yuan, 1981. Induction of haploid rice plantlets by ovary culture. Plant Sci. Lett. 20: 231–237.

    Google Scholar 

  • Chin-chin, Chu., 1978. The N6 medium and its applications to anther culture cereal crops. Proceedings of Symposium on Plant Tissue Culture, 1978, 43–50.

  • Cocking, E.C., 1960. A method for the isolation of plant protoplast and vacuoles. Nature, 187: 962–963.

    Google Scholar 

  • Conger, B.U., G.E., Hanning, D.J., Gray & J.K., McDaniel, 1983. Direct embryogenesis from mesophyll cells of orchadgrass. Science, 221: 850–851.

    Google Scholar 

  • Devay, M.R., J.P. Jotham, D.M. Glimour, T.K. Ghose & J.J. Rybczyński, 1986. The development of protoplast systems suitable for gene transfer in forage Legumes. In: Plant Tissue Culture and its Agricultural Application. Ed: Withers L.A. and T. Alderson, 1986, 337–348.

  • Deka, P.C. & S.K., Sen, 1976. Differentiation in calli originating from isolated protoplasts of rice (Oryza sativa) through plating technique. Mol. Gen. Genet., 145: 239–244.

    Google Scholar 

  • De la, Pena, A., H., Lörz & J., Schell, 1986. Transgenic rye plants obtained by injection of DNA into young floral tillers. Nature, 6101: 274–276.

    Google Scholar 

  • De la, Roche, A.J., W.A., Keller, J., Singh & D., Siminovitch, 1977. Isolation of protoplasts from unhardened and hardened tissues of winter rye and wheat. Can. J. Bot. 55: 1181–1185.

    Google Scholar 

  • De, Ropp, R.S., 1945. Studies on the physiology of leaf growth I. Am. Bot. NS., 9: 369–381.

    Google Scholar 

  • Dudits, D., G. Hadlaczky, E. Levi, O. Fejer, Z. Haydu & O. Lazar, Somatic hybridization of Daucus carota and D. cappilifolius by protoplast fusion. Theor. Appl. Genet., 51: 127–132.

  • Eapen, S. & P.S., Rao, 1982. Callus induction and plant regeneration from immature embryos of rye and triticale. Plant Cell Tissue Organ Culture, 1: 221–227.

    Google Scholar 

  • Eapen, S. and P.S., Rao, 1984. Factors controlling callus proliferation, growth and regeneration from immature embryos of rye and triticale. Proc. Ind. Nat. Sci. Acad. B 50, 4: 431–437.

    Google Scholar 

  • Eapen, S. & P.S., Rao, 1985. Plant regeneration from immature inflorescense callus culture of wheat, rye and triticale. Euphytica, 34: 153–159.

    Google Scholar 

  • Friedt, W., V., Lind, H., Walther, B., Foroughi-Wehr, S., Zuchner & G., Wenzel, 1983. The value of inbred lines of derived from Secale cereale x S. vavilovii via classical inbreeding and androgenic haploids. Z. Pflanzenzücht, 91: 89–103.

    Google Scholar 

  • Gamborg, O.L., R.A., Muller & K., Ojimak, 1968. Nutrient requirements of suspension culture of soybean root cells. Exp. Cell. Res., 50: 151–158.

    Google Scholar 

  • Gamborg, O.L., F., Constabel & R.A., Miller, 1970. Embryogenesis and production of albino plants from cell cultures of Bromus inermis. Planta, 95: 355–358.

    Google Scholar 

  • Guha, S. & S.C., Maheshwari, 1964. In vitro production of embryos from anthers of Datura. Nature, 204: 497.

    Google Scholar 

  • Guha, S. & S.C., Maheshwari, 1966. Cell division and diferentiation of embryos in the pollen grains of Datura in vitro. Nature, 212: 97–98.

    Google Scholar 

  • Guha, S. & S.C., Maheashwari, 1967. Development of embryoids from grains of Datura in vitro. Phytomorphology, 17: 454–461.

    Google Scholar 

  • Haccius, B., 1978. Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology, 28: 74–81.

    Google Scholar 

  • Heller, R., 1953. Recherches sur la nutrition minerale des tissues végétaux cultivés in vitro. Ann. Sce. Nat. Bot. et Biol. Veg., 14: 1–223.

    Google Scholar 

  • Hoffmann, F., 1978. Mutation and selection of haploid cell culture systems of rape and rye. In Proceedings of an International Symposium of Plant Cell Culture ‘Production of natural compounds of cell culture methods’ pp 319–329.

  • Hoffmann, F. & G., Wenzel, 1981. Self compatibility in microspore-derived doubled-haploid rye lines and single grain selection for alkylresorcinal content. Theor. Appl. Gen., 60: 129–133.

    Google Scholar 

  • Jones, M.G.K. and P.J., Dale, 1982. Reproducible regeneration of callus from suspensicon culture protoplasts of the grass Lolium multiflorum. Z. Pflanzenphysiol., 105: 267–274.

    Google Scholar 

  • Krumbiegel-Schroern, G., J., Finger, V., Schroeren & H., Binding, 1984. Embryoid formation and plant regeneration from callus of Secale cereale L. Z. Pflanzenzüchtg., 92: 89–94.

    Google Scholar 

  • Kunert, R., 1979. The effect of gibberellic acid on the development of unvernalized winter rye plants. Tag. Ber. Akad. Landwirtsch-Wiss. DDR, 168: 217–299.

    Google Scholar 

  • Lin, M. & J., Staba, 1961. Peppermint and spearmint tissue cultures callus formation and submerged culture. Lloydia, 24: 139–145.

    Google Scholar 

  • Linsmaier, E.M. & F., Skoog, 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plantarum, 18: 100–127.

    Google Scholar 

  • Linacero, R. & A.M. Vazquez, 1986. Somacloncal variation in plants regenerated from embryo calluses in rye (Secale cereale L.). Proceedings International Symposium organized by Eucarpia. Sept., 8–13, 1985,. Berlin (West). Ed. W., Horn et al., 479–481.

  • Linacero, R. & A.M., Vazquez, 1986. Somatic embryogenesis and plant regeneration from leaf tissue of rye (Secale cereale L.). Plant Science, 44: 219–222.

    Google Scholar 

  • Lu, C., S.F., Chandler & I.K., Vasil, 1984. Somatic embryogenesis and plant regeneration from cultured immature embryos of rye (Secale cereale L.). J. Plant Physiol., 115: 237–244.

    Google Scholar 

  • Malepszy, S., 1973. Callus formation by Secale cereale L. anthers. Haploid Information Service, 7: 2–3 (in German).

    Google Scholar 

  • Malepszy, S., 1975. A contribution to the production of haploids in rye (Secale cereale L.). Bull. de L'Acad. Pol. des Sciences CLII, 23: 167–172.

    Google Scholar 

  • Mottley, J. and J., Sybenga, 1988. In vitro culture of cell aggregates of rye (Secale cereale L.) at low densities. Plant Cell Reports, 7: 193–196.

    Google Scholar 

  • Mulfin, M., 1970. Tissue culture of some monocotyledonous plants. Austr. J. Biol. Sciences. 23: 473–477.

    Google Scholar 

  • Murashige, T. & F., Skoog, 1962. A revised medium for rapid growth and bioassays with tabacco tissue culture. Physiol. Plant., 15: 473–497.

    Google Scholar 

  • Nitsch, J.P. & C., Nitsch, 1969. Haploid plant from pollen grain. Sciences 163: 85–87.

    Google Scholar 

  • Nitzsche, W. & G., Wenzel, 1977. Haploids in plant breeding. Fortschrift der Pflanzenzüchtung 8: 38–42.

    Google Scholar 

  • Norstog, K.S., 1956. Growth of ryegrass endosperm in vitro. Bot. Gaz. 117: 253–259.

    Google Scholar 

  • Norstog, K.S., 1967. Studies of the survival of very small barley embryos in culture. Bull. Torrey Bot. Club. 94: 223–229.

    Google Scholar 

  • Orlikowska, T., 1977. Induction of androgenesis in vitro in Secale cereale and Triticale. Genet. Pol., 18: 51–59.

    Google Scholar 

  • Pfahler, P.L., 1965. In vitro germination of rye (Secale cereale L.) pollen. Crop Sciences, 5: 597–598.

    Google Scholar 

  • Pojnar, E., 1980. Rye protoplasts isolation and culture. Gen. Pol., 21: 243–245.

    Google Scholar 

  • Potrykus, I., C.T. Harms & H. Lörz, 1976. Problems in culturing cereal protoplasts. in: ‘Cell Genetics in Higher Plants’, Ed. Dudits et al., 129–140.

  • Potrykus, I., C.T., Harms & H., Lörz: 1979. Callus formation from cell culture protoplasts of (Zea mays). Theor. Appl. Genet., 54: 209–214.

    Google Scholar 

  • Roberts, E. & H.E., Street, 1955. The continuous culture of excised rye roots. Physiol. Plant., 8: 238–262.

    Google Scholar 

  • Rueda, J. & A.M., Vazquez, 1985. Effect of auxins and cytokinin up on the start of meiosis in cultured anthers of rye (Secale cereale L.). Can. J. Gen. and Cyt., 27: 759–765.

    Google Scholar 

  • Rybczyński, J.J., 1978. In vitro culture of mature rye (Secale cereale L.) embryo fragments. Gen. Pol., 19: 241–255.

    Google Scholar 

  • Rybczyński, J.J., 1978. Effect of 2.4-D on callus formation and rhizogenesis in vitro culture of the immature embryo scutellum of di- and tetraploid rye (Secale cereale L.). Gen. Pol., 19: 467–485.

    Google Scholar 

  • Rybczyński, J.J., 1979. The influence of cytokinin (BAP, KIN, ZEAT) on the processes on callusing and caulogenesis of the immature embryo scutellum of di- and tetraploid rye (Secale cereale L.). Gen. Pol., 20: 11–24.

    Google Scholar 

  • Rybczyński, J.J., 1980. In vitro culture of Secale cereale L. explants-callus formation and organ differentiation. Acta Soc. Bot. Pol., 49: 155–160.

    Google Scholar 

  • Rybczyński, J.J., A., Stolarz & A., Slusarkiewicz-Jarzina, 1980. Differentiation of shoot elements from rachis of Secale cereale L. Acta Soc. Bot. Pol., 49: 161–68.

    Google Scholar 

  • Rybczyński, JJ., Z., Zwierzykowski & A., Slusarkiewicz-Jarzina, 1983. Plant regeneration with doubled chromosome number in tissue culture of F1 Lolium-Festuca hybrids. Gen. Pol., 24: 1–8.

    Google Scholar 

  • Rybczyński, J.J., & J. Zimny, 1985. Screening of cultivar varieties of Secale cereale L. in order to obtain somatic embryogenesis. Proceedings of Eucarpia Meeting of the Cereal Section on Rye, Svalov, June 11–13, 1985, 33–53.

  • Rybczyński, J.J., & W., Zduńczyk, 1986. Somatic embryogenesis and plantlet regeneration in the genus Secale. 1. Somatic embryogenesis and organogenesis from immature embryos of five wild species of rye. Theor. Appl. Genet., 73: 267–271.

    Google Scholar 

  • Salbach, G., & H., Koblitz, 1978. Attempts to initiate callus formation from barley leaves. Plant Science Lett., 13: 165–169.

    Google Scholar 

  • Sarhan, F., & D., Cesar, 1988. High yield of mesophyll protoplasts from wheat, barley and rye. Physiol. Plant. 72: 337–342.

    Google Scholar 

  • Sheridan, W.F., 1974. Tissue culture of wheat rye and their hybrid. Wheat Information Service, 38: 8–12.

    Google Scholar 

  • Shiminovitch, D., J., Singh & J.A.de La, Roche, 1978. Freezing behaviour of free protoplasts of winter rye. Cryobiology 15: 205–213.

    Google Scholar 

  • Singh, J., 1979. Freezing of protoplasts isolated from coldhardened and non-hardened winter rye. Plant Sci. Lett. 16: 195–201.

    Google Scholar 

  • Stefaniak, B. 1984. Development of rye embryo. I. Preliminary investigations on ultrastructure of 7-day-old proembryo. Acta Soc. Bot. Pol. 53: 11–16.

    Google Scholar 

  • Stefaniak, B., 1984. In vitro culture of undifferentiated proembryos of rye. Proceedings of International Symposium ‘Plant Tissue and Cell Culture Application to Crop Improvement’ Olomonc. Sept. 24–29th,, 1984, 111–112.

  • Stefaniak, B., 1987. The in vitro development of isolated rye proembryo. Acta Soc. Bot. Pol., 56: 37–42.

    Google Scholar 

  • Stolarz, A., 1974. Induction of androgenesis in Secale cereale var. Strzekacinskie pollen grains in ‘in vitro’ conditions. Plant Breeding, Acclimatization and Seeds Production, 18: 217–220 (in Polish).

    Google Scholar 

  • Stolarz, A., 1977. Initital stages of androgenesis in Secale cereale L. in the ‘in vitro’ anther culture, in: ‘Use of Tissue Cultures in Plant Breeding’. Proceedings of the International Symposium, 6–11 Sept. 1976, Olomouc, Ed. F. Novak: 381–388.

  • Sulinowski, S., K. Sekowska, H. Sadowska & J.J. Rybczyński, 1976. Mejotic triploids and tetraploids of Secale cereale. In Abstracts of VI National Congress of Polish Genetics Association, Bialystok, 10–12 Sept. (in Polish): 55–56.

  • Sybenga, J., 1985. Genetics and Biotechnology. Proceeding of Eucarpia Meeting of the Cereal Section on rye. Svalov, June 11–13th, 1985: 11–30.

  • Thomas, E., F., Hoffmann & G., Wenzel, 1975. Haploid plantlets from microspores of rye. Z. Pflanzenzüchtg. 75: 106–113.

    Google Scholar 

  • Thomas, E., F., Hoffmann & G., Wenzel, 1977. Callus and cell suspensions from flower stem heades of haploid Secale cereale. Haploid Information Service, 18: 14–16.

    Google Scholar 

  • Vasil, I.K., 1983. Regeneration of plants from single cells of cereals and grasses. In ‘Genetic Engineering in Eukaryotes’. Ed. Larquin, F. and Kleinhofs, A., 233–251.

  • Vences, F.J., F., Vaquero, A.M., Vazquez & M. Perezade la, Vega, 1986. Izoenzymatic changes during in vitro morphogenetic processes in self pollinated species of Secale. J. Plant Physiol., 123: 31–36.

    Google Scholar 

  • Wakasa, K., 1973. Isolation of protoplasts from plant organs Jap. J. Gen., 48: 279–289.

    Google Scholar 

  • Wenzel, G., 1973. Isolation of leaf protoplasts from haploid plants of petunia, rape and rye. Z. Pflanzenzüchtg., 69, 58–61.

    Google Scholar 

  • Wenzel, G. and E., Thomas, 1974. Observations of the growth in culture of anthers of Secale cerealeL. Z. Pflanzenzüchtg.: 72, 89–94.

    Google Scholar 

  • Wenzel, G., F., Hoffmann & E., Thomas, 1976. He microspore-derived plants in rye. Theor. Appl. Gen. 8: 205–208.

    Google Scholar 

  • Wenzel, G., F., Hoffman, I., Potrykus & E., Thomas, 1975. The separation of viable rye microspores from mixed populations and their development in culture. Mol. Gen. Gen. 138: 93–297.

    Google Scholar 

  • Wenzel, G., F., Hoffmann & E., Thomas, 1977. Increased induction and chromosome doubling of androgenetic haploid rye. Theor. Appl. Gen., 51: 81–86.

    Google Scholar 

  • White, P.R., 1954. The cultivation of animal and plant cell, Ronald Press. Co., N.Y.

    Google Scholar 

  • Zenkteler, M. & E., Misiura, 1974. Induction of androgenic embryos from cultured anthers of Hordeum, Secale and Festuca, B. Physiol. Pflanzen. (BPP), 165: 337–340.

    Google Scholar 

  • Zenkteler, M. & W., Nitzsche, 1984. Wide hybridization experiments in cereals. Theor. Appl. Gen. 68: 311–315.

    Google Scholar 

  • Zimny, J. & H. Lörz, 1986. Somatic embryogenesis and plant regeneration from meristematic tissue of Secale cereale L. rye. Genetic Manipulation in Plant Breeding. Proceedings International Symposium, Eucarpia, Sept. 8–13, 1985 Berlin (West) Germany, 503–505.

  • Yang, D.P. & E.U., Dodson, 1970. The effect of kinetin on the growth of diploid and tetraploid root tips of rye in vitro. Can. J. Bot. 48: 19–25.

    Google Scholar 

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  1. Plant Tissue Culture Laboratory, The Botanical Garden of the Polish Academy of Sciences, ul Prawdziwka 2, 02-973, Warsaw, Poland

    Jan J. Rybczyński

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Rybczyński, J.J. Plant tissue culture of Secale: A review. Euphytica 46, 57–70 (1990). https://doi.org/10.1007/BF00057619

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