Abstract
The sequence determination of several genomic clones isolated from the Mediterranean fruitfly Ceratitis capitata identified the existence of opa-like repeats, often more than one being clustered in small chromosomal segments. These repeats have previously been shown to consist of stretches of tandemly reiterated glutamine-encoding residues, and they are found in multiple genes of several organisms. Most of the repeats described here are flanked or interrupted by stop codons in all reading frames and, thus, could not possibly be part of protein-coding sequences. Furthermore, these repeats, of which there are several hundred in the genome of the Medfly, can be used effectively for the determination of sequence polymorphisms, providing a convenient approach to obtain additional landmarks for the construction of genomic maps of this economically important insect.
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AltschulS. F., W.Gish, W.Miller, E. W.Myers & D. J.Lipman, 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.
Artavanis-TsakonasS., 1988. The molecular biology of the Notch locus and the fine tuning of differentiation in Drosophila. Trends Genet. 4:95–100.
AshburnerM., 1992. Mapping insect genomes, pp. 51–75 Insect Molecular Science, edited by J. M.Crampton and P.Eggleston. Academic Press, London.
Bellané-ChantelotC., B.Lacroix, P.Ougen, A.Billault, S.Beaufils, S.Bertrand, I.Georges, F.Glibert, I.Gros, G.Lucotte, L.Susini, J.-J.Codani, P.Gesnoin, S.Pook, G.Veysseix, J.Le-Kuo, T.Ried, D.Ward, I.Chumakov, D.LePaslier, E.Barillot & D.Cohen, 1992. Mapping the whole human genome by fingerprinting yeast artifical chromosomes. Cell 70: 1059–1068.
BeloteJ. M., M.McKeon, R. T.Boggs, R.Ohkawa & B. A.Sosnowski, 1989. The molecular genetics of transformer, a genetic switch controlling sexual differentiation in Drosophila. Devel. Genet. 10: 237–245.
BrookJ. D., M. E.McCurrach, H. G.Harley, A. J.Buckler, D.Church, H.Aburatani, K.Hunter, V. P.Stanton, J.-P.Thirion, T.Hudson, R.Sohn, B.Zemelman, R. G.Snell, S. A.Rundle, S.Crow, J.Davies, P.Shelbourne, J.Buxton, C.Jones, V.Juvonen, K.Johnson, P. S.Harper, D. J.Shaw & D. E.Housman, 1992. Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3′ end of a transcript encoding a protein kinase family member. Cell 68: 799–808.
CangianoG., H.Ameer, R.Waterston & A.LaVolpe, 1990. Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans. Nucl. Acids. Res. 18: 5077–5081.
ChirgwinJ. J., A. E.Przbyla, R. J.MacDonald & W. J.Rutter, 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299.
DevereuxJ., P.Haeberli & O.Smithies, 1984. A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids. Res. 12: 387–395.
DietrichW., H.Katz, S. E.Lincoln, H.-S.Shin, J.Friedman, N. C.Dracopoli & E. S.Lander, 1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131: 423–447.
GrabowskiD. T., J. P.Carney & M. R.Kelley, 1991. A Drosophila gene containing the opa repetitive element is exclusively expressed in adult male abdomens. Nucl. Acids Res. 19: 1709.
HigginsD. G. & P. M.Sharp, 1988. CLUSTAL; a package for performing multiple sequence alignment on a microcomputer. Gene 73: 237–244.
Huntington's disease collaborative research group, 1993. A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. Cell 72: 971–983.
KeyesL. N., T. W.Cline & P.Schedl, 1992. The primary sex determination signal of Drosophila acts at the level of transcription. Cell 68: 933–943.
PearsonW. R. & D. J.Lipman, 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85: 2444–2448.
RinaM. & C.Savakis, 1991. A cluster of vitellogenin genes in the Mediterranean fruit fly Ceratitis capitata: sequence and structural conservation in dipteran yolk proteins and their genes. Genetics 127: 769–780.
SambrookJ., E. F.Fritsch & T.Maniatis, 1989. Molecular cloning: A laboratory manual; Cold Spring Harbor Laboratory Press, 3 Vols., Cold Spring Harbor, N.Y.
ScottM. P., J. W.Tamkun & G. W.HartzellIII 1989. The structure and function of the homeodomain. Biochim. Biophys. Acta. 989: 25–48.
StadenR., 1984. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 12: 521–538.
TautzD., 1989. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl. Acids. Res. 17: 6463–6471.
WallaceR. B., M. J.Johnson, S. Y.Suggs, K.Miyoshi, R.Bhatt & K.Itakura, 1981. A set of synthetic oligodeoxyrlbonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene 16: 21–26.
WhartonK. A., B.Yedvobnick, V. G.Finerty & S.Artavanis-Tsakonas, 1985. opa: a novel family of transoribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster. Cell 40: 55–62.
YedvobnickB., D.Smoller, P.Young & D.Milis, 1988. Molecular analysis of the neurogenic locus mastermind of D. melanogaster. Genetics 118: 483–497.
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This paper is dedicated to the memory of our colleague and friend Dr. Jim Flach who took part in the initial phase of this work and died during the course of the investigation.
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Sidén-Kiamos, I., Favia, G., Artiaco, D. et al. Opa-like repeats in the genome of the Medfly Ceratitis capitata . Genetica 92, 43–53 (1993). https://doi.org/10.1007/BF00057506
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DOI: https://doi.org/10.1007/BF00057506