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Opa-like repeats in the genome of the Medfly Ceratitis capitata

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Abstract

The sequence determination of several genomic clones isolated from the Mediterranean fruitfly Ceratitis capitata identified the existence of opa-like repeats, often more than one being clustered in small chromosomal segments. These repeats have previously been shown to consist of stretches of tandemly reiterated glutamine-encoding residues, and they are found in multiple genes of several organisms. Most of the repeats described here are flanked or interrupted by stop codons in all reading frames and, thus, could not possibly be part of protein-coding sequences. Furthermore, these repeats, of which there are several hundred in the genome of the Medfly, can be used effectively for the determination of sequence polymorphisms, providing a convenient approach to obtain additional landmarks for the construction of genomic maps of this economically important insect.

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References

  • AltschulS. F., W.Gish, W.Miller, E. W.Myers & D. J.Lipman, 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403–410.

    Google Scholar 

  • Artavanis-TsakonasS., 1988. The molecular biology of the Notch locus and the fine tuning of differentiation in Drosophila. Trends Genet. 4:95–100.

    Google Scholar 

  • AshburnerM., 1992. Mapping insect genomes, pp. 51–75 Insect Molecular Science, edited by J. M.Crampton and P.Eggleston. Academic Press, London.

    Google Scholar 

  • Bellané-ChantelotC., B.Lacroix, P.Ougen, A.Billault, S.Beaufils, S.Bertrand, I.Georges, F.Glibert, I.Gros, G.Lucotte, L.Susini, J.-J.Codani, P.Gesnoin, S.Pook, G.Veysseix, J.Le-Kuo, T.Ried, D.Ward, I.Chumakov, D.LePaslier, E.Barillot & D.Cohen, 1992. Mapping the whole human genome by fingerprinting yeast artifical chromosomes. Cell 70: 1059–1068.

    Google Scholar 

  • BeloteJ. M., M.McKeon, R. T.Boggs, R.Ohkawa & B. A.Sosnowski, 1989. The molecular genetics of transformer, a genetic switch controlling sexual differentiation in Drosophila. Devel. Genet. 10: 237–245.

    Google Scholar 

  • BrookJ. D., M. E.McCurrach, H. G.Harley, A. J.Buckler, D.Church, H.Aburatani, K.Hunter, V. P.Stanton, J.-P.Thirion, T.Hudson, R.Sohn, B.Zemelman, R. G.Snell, S. A.Rundle, S.Crow, J.Davies, P.Shelbourne, J.Buxton, C.Jones, V.Juvonen, K.Johnson, P. S.Harper, D. J.Shaw & D. E.Housman, 1992. Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3′ end of a transcript encoding a protein kinase family member. Cell 68: 799–808.

    Google Scholar 

  • CangianoG., H.Ameer, R.Waterston & A.LaVolpe, 1990. Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans. Nucl. Acids. Res. 18: 5077–5081.

    Google Scholar 

  • ChirgwinJ. J., A. E.Przbyla, R. J.MacDonald & W. J.Rutter, 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299.

    Google Scholar 

  • DevereuxJ., P.Haeberli & O.Smithies, 1984. A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids. Res. 12: 387–395.

    Google Scholar 

  • DietrichW., H.Katz, S. E.Lincoln, H.-S.Shin, J.Friedman, N. C.Dracopoli & E. S.Lander, 1992. A genetic map of the mouse suitable for typing intraspecific crosses. Genetics 131: 423–447.

    Google Scholar 

  • GrabowskiD. T., J. P.Carney & M. R.Kelley, 1991. A Drosophila gene containing the opa repetitive element is exclusively expressed in adult male abdomens. Nucl. Acids Res. 19: 1709.

    Google Scholar 

  • HigginsD. G. & P. M.Sharp, 1988. CLUSTAL; a package for performing multiple sequence alignment on a microcomputer. Gene 73: 237–244.

    Google Scholar 

  • Huntington's disease collaborative research group, 1993. A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes. Cell 72: 971–983.

    Google Scholar 

  • KeyesL. N., T. W.Cline & P.Schedl, 1992. The primary sex determination signal of Drosophila acts at the level of transcription. Cell 68: 933–943.

    Google Scholar 

  • PearsonW. R. & D. J.Lipman, 1988. Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85: 2444–2448.

    Google Scholar 

  • RinaM. & C.Savakis, 1991. A cluster of vitellogenin genes in the Mediterranean fruit fly Ceratitis capitata: sequence and structural conservation in dipteran yolk proteins and their genes. Genetics 127: 769–780.

    Google Scholar 

  • SambrookJ., E. F.Fritsch & T.Maniatis, 1989. Molecular cloning: A laboratory manual; Cold Spring Harbor Laboratory Press, 3 Vols., Cold Spring Harbor, N.Y.

    Google Scholar 

  • ScottM. P., J. W.Tamkun & G. W.HartzellIII 1989. The structure and function of the homeodomain. Biochim. Biophys. Acta. 989: 25–48.

    Google Scholar 

  • StadenR., 1984. Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res. 12: 521–538.

    Google Scholar 

  • TautzD., 1989. Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl. Acids. Res. 17: 6463–6471.

    Google Scholar 

  • WallaceR. B., M. J.Johnson, S. Y.Suggs, K.Miyoshi, R.Bhatt & K.Itakura, 1981. A set of synthetic oligodeoxyrlbonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene 16: 21–26.

    Google Scholar 

  • WhartonK. A., B.Yedvobnick, V. G.Finerty & S.Artavanis-Tsakonas, 1985. opa: a novel family of transoribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster. Cell 40: 55–62.

    Google Scholar 

  • YedvobnickB., D.Smoller, P.Young & D.Milis, 1988. Molecular analysis of the neurogenic locus mastermind of D. melanogaster. Genetics 118: 483–497.

    Google Scholar 

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This paper is dedicated to the memory of our colleague and friend Dr. Jim Flach who took part in the initial phase of this work and died during the course of the investigation.

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Sidén-Kiamos, I., Favia, G., Artiaco, D. et al. Opa-like repeats in the genome of the Medfly Ceratitis capitata . Genetica 92, 43–53 (1993). https://doi.org/10.1007/BF00057506

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