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Evaluation of a model for the effects of substrate interactions on the kinetics of reductive dehalogenation

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Abstract

The effects of primary electron-donor and electron-acceptor substrates on the kinetics of TCA biodegradation in sulfate-reducing and methanogenic biofilm reactors are presented. Of the common anaerobic electron-donor substrates that were tested, only formate stimulated the TCA biodegradation rate in both reactors. In the sulfate-reducing reactor, glucose also stimulated the reaction rate. The effects of formate and sulfate on TCA biodegradation kinetics were analyzed using a model for primary substrate effects on reductive dehalogenation. Although some differences between the model and the data are evident, the observed responses of the TCA degradation rate to formate and sulfate were consistent with the model. Formate stimulated the TCA degradation rate in both reactors over the entire range of TCA concentrations that were studied (from 50 μg TCA/L to 100 mg TCA/L). The largest effects occurred at high TCA concentrations, where the dehalogenation kinetics were zero order. Sulfate inhibited the first-order TCA degradation rate in the sulfate-reducing reactor, but not in the methanogenic reactor. Molybdate, which is a selective inhibitor of sulfate reduction, stimulated the TCA removal rate in the sulfate-reducing reactor, but had no effect in the methanogenic reactor.

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  1. Department of Civil and Environmental Engineering, University of Cincinnati, 45221, Cincinnati, OH, USA

    Brian A. Wrenn

  2. Department of Civil Engineering, Northwestern University, 60208-3109, Evanston, IL, USA

    Bruce E. Rittmann

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  1. Brian A. Wrenn
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Wrenn, B.A., Rittmann, B.E. Evaluation of a model for the effects of substrate interactions on the kinetics of reductive dehalogenation. Biodegradation 7, 49–64 (1996). https://doi.org/10.1007/BF00056558

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