Abstract
A new purification procedure for spinach leaf fructose-1,6-bisphosphatase is proposed, which includes the use of affinity chromatography on mercaptoethylamine-Sepharose. A homogeneous preparation of the enzyme can be obtained in 48 hr, with a specific activity of 67 U/mg and a yield of 23%. The method may also be useful for the purification of other thioredoxin-activated chloroplast enzymes.
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Plá, A., Chueca, A. & López-Gorgé, J. A new procedure for the purification of spinach leaf photosynthetic fructose-1,6-bisphosphatase by affinity chromatography on mercaptoethylamine-Sepharose. Photosynth Res 2, 291–296 (1981). https://doi.org/10.1007/BF00056266
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DOI: https://doi.org/10.1007/BF00056266