Abstract
Three carbohydrate sources, maltose, sucrose and cellobiose, were compared for the respective effects on regeneration from internodal sections of flax. It was found that different patterns of morphogenesis were attributable to the type of carbohydrate and its concentration. Mean shoot regeneration was highest on a medium containing 87 mM maltose with a rate of 2.4 shoots per explant and root formation was stimulated to the greatest degree on a medium with 87 mM sucrose. This medium also resulted in the highest tissue dry weight.
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Babbar SB & Gupta SC (1986) Effect of carbon source on Datura metel microspore embryogenesis and the growth of callus raised from microspore-derived embryos. Biochem. Physiol. Pflanzen 181: 331–338
Barakat MN & Cocking EC (1983) Plant regeneration from protoplast-derived tissues of Linum usitatissimum L. Plant Cell Rep. 2: 314–317
Basiran N, Armitage P, Scott RJ & Draper J (1987) Genetic transformation of flax by Agrobacterium tumefaciens. Plant Cell Rep. 6: 396–399
Bennet MD & Smith JB (1976) Nuclear DNA amounts in angiosperms. Proc. Roy Soc. Lond. B. 274: 227–274
De Lange JH (1989) Significance of autoclaving-induced toxicity from and hydrolysis of carbohydrates in in vitro studies of pollen germination and tube growth. S. Afr. J. Bot. 55: 1–5
Finnie SJ, Powell W & Dyer AF (1989) The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.) Plant Breeding 103: 110–118
Galiba G & Erdei LS (1986) Dependence of wheat callus growth differentiation and mineral content on carbohydrate supply. Plant Sci. 45: 65–70
Gamborg OL & Shyluk JP (1976) Tissue culture, protoplasts and morphogenesis in flax. Bot Gaz. 137: 301–06
Gross KC, Pharr DM & Locy RD (1981) Growth of callus initiated from cucumber hypocotyls on galactose and galactose-containing oligosaccharides. Plant Sci. Lett. 20: 333–341
Hildebrandt AC & Riker AJ (1949) The influence of various carbon components on the growth of marigold, paris daisy, periwinkle, sunflower and tobacco tissue in vitro. Amer. J. Bot. 36: 74–85
Ibrahim RK (1971) Media for the growth of flax tissue culture, protoplasts and morphogenesis. Can. J. Bot. 49: 295–298
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nickell LG & Maretzki A (1970) The utilization of sugars and starch as carbon sources by sugarcane cell suspension cultures. Plant Cell Physiol. 11: 183–185
Raquin C (1983) Utilization of different sugars as a carbon source for in vitro anther culture of Petunia. Z. Pflanzenphysiol. 111: 453–457
Redenbaugh K, Viss P, Slade D & Fujii J (1987) Scale-up: Artificial seeds. In: Green CE, Somers DA, Hackett WP & Biesboer DD (Eds) Plant Tissue and Cell Culture (pp 473–493). Alan R. Liss Inc, New York
Strickland SG, Nichol JW, McCall CM & Stuart DA (1987) Effect of carbohydrate source on alfafa somatic embryogenesis. Plant Sci. 48: 113–121
Verma DC & Dougall DK (1977) Influence of carbohydrates on quantitative aspects of growth and embryo formation in wild carrot suspension cultures. Plant Physiol. 59: 81–85
Vuke TM & Mott RL (1987) Growth of loblolly pine callus on a variety of carbohydrate sources. Plant Cell Rep. 6: 153–156
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Millam, S., Davidson, D. & Powell, W. The use of flax (Linum usitatissimum) as a model system for studies on organogenesis in vitro: the effect of different carbohydrates. Plant Cell Tiss Organ Cult 28, 163–166 (1992). https://doi.org/10.1007/BF00055512
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DOI: https://doi.org/10.1007/BF00055512