Abstract
Callus cultures were established from hypocotyl explants of R. bracteosa, R. chalepensis and R. macrophylla. Calli were maintained for more than three years on MS-medium supplemented with 1 mg l-1 of each 2,4-D and kinetin. Acridone and furoquinoline alkaloids and coumarins have been isolated from four week old calli grown on a hormone containing and hormone-free medium. A new chlorinated acridone alkaloid has been detected.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
medium after Murashige & Skoog [6]
References
Baumert A, Gröger D, Schmidt J & Mügge C (1987) Minor alkaloids from Ruta graveolens tissue cultures. Pharmazie 42: 67–68
Baumert A, Gröger D, Schmidt J, Kuzovkina IN & Mügge C (1988) Alkaloids and other constituents from tissue cultures of Ruta graveolens. Fitoterapia 59: 83–88
Eilert U, Engel B, Reinhard E & Wolters B (1983) Acridone epoxides in cell cultures of Ruta species. Phytochemistry 22: 14–15
Hegnauer R (1983) Chemical characters and the classification of the Rutales. In: Waterman PG & Grundon MF (Eds) Chemistry and Chemical Taxonomy of the Rutales. (pp 401–440). Academic Press, London
Mester I (1983) Structural diversity and distribution of alkaloids in the Rutales. In: Waterman PG & Grundon MF (Eds) Chemistry and Taxonomy of the Rutales (pp 31–96). Academic Press, London
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nahrstedt A, Wray V, Engel B & Reinhard E (1985) New furoacridone alkaloids from tissue culture of Ruta graveolens. Planta Med. 517–519
N.N. (1971) Chemistry and biology of Rutaceae compounds. International Symposium Herba Hungarica 10, No 2–3: 7–145
Petit-Paly G, Ramawat KG, Chenieux IC & Rideau M (1989) Ruta graveolens: in vitro production of alkaloids and medicinal compounds. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry, Vol 7. Medicinal and aromatic plants (pp 488–505) Springer, Berlin Heidelberg
Reinhard R, Corduan G & Volk OH (1968) Über Gewebekulturen von Ruta graveolens. Planta Med. 16: 8–16
Reisch J & Gunaherath GMK (1989) Natural products chemistry Part 124. Revised structure and synthesis of a new acridone alkaloid. Hallacridone from Ruta graveolens tissue cultures. J. Chem. Soc. Perkin Trans. I 1047–1051
Rosza Zs, Reisch J, Szendrei K & Minker E (1981) Rutacridone-epoxide and gravacridonol from the roots of Ruta graveolens. Fitoterapia 52: 93–95
Scharlemann W (1972) Acridin-Alkaloide aus Kallus Kulturen von Ruta graveolens L. Z. Naturforsch. 27b: 806–809
White PhR (1943) A Handbook of the Plant Tissue Culture. The Ronald Press Company, New York
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Baumert, A., Gröger, D., Kuzovkina, I.N. et al. Secondary metabolites produced by callus cultures of various Ruta species. Plant Cell Tiss Organ Cult 28, 159–162 (1992). https://doi.org/10.1007/BF00055511
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DOI: https://doi.org/10.1007/BF00055511