Abstract
Utilizing a two-beam technique in the frequency domain, the pumped absorption of PS II membrane fragments from spinach and of acetonic chlorophyll-a solutions was measured at room temperature. In a very narrow wavelength region (0.2 nm around the pump pulse wavelength) the relative test beam transmission exhibited either a decrease or an increase, respectively, dependent on the intensity of a strong pump beam. In contrast, the transmission changes of chl-a solutions were not affected by the wavelength mistuning between pump and test beam. The data obtained for PS II membrane fragments were interpreted in terms of excited state absorption of pigment-protein clusters within the light-harvesting complex of PS II. The interpretation of the small absorption band as a homogeneously broadened line led to a transversal relaxation time for chlorophyll in vivo of about 1 ps.
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Abbreviations
- PS I:
-
photosystem I of green plants
- PS II:
-
photosystem II of green plants
- P700:
-
primary donor of PS I
- P680:
-
primary donor of PS II
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Bittner, T., Voigt, J., Kehrberg, G. et al. Evidence of excited state absorption in PS II membrane fragments. Photosynth Res 28, 131–139 (1991). https://doi.org/10.1007/BF00054126
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DOI: https://doi.org/10.1007/BF00054126