Summary
5-hydroxytryptamine (5HT) treatment produced dose-related contractions in the human internal mammary artery with an EC50 value of 3.4×10-7M. The 5HT2 receptor antagonist ketanserin reversed the contractions evoked by 5HT in a competitive manner at a low concentration (10-6 M), whereas a noncompetitive antagonism was apparent at higher concentrations (5× 10-8 M to 5×10-7 M). The alpha1-blocking component of ketanserin was evaluated by studying the effect of ketanserin upon the contractile response evoked by norepinephrine. Up to 10-7 M, ketanserin did not influence norepinephrine-induced contractions. These findings indicate that the mammary artery is a vascular tissue sensitive to contractions induced by 5HT and that the drug ketanserin antagonizes this contractile response throuth the 5HT2 receptor subtype.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burrows ME, Vanhoutte PM. Pharmacology of arterioles: Some aspects of variability in response to norepinephrine, histamine and 5-hydroxytryptamine. J Cardiovasc Pharmacol 1981;3:1370–1380.
Vanhoutte PM. Cardiovascular effects of serotonin. J Cardiovasc Pharmacol 1987;10 (Suppl 3):S8-S11.
Houston D, Vanhoutte PM. Serotonin and the vascular system: Role in health and disease and implication for therapy. Drugs 1986;31:149–163.
Breckenridge A. Ketanserin, a new antihypertensive agent. J Hypertens 1986;4 (Suppl 1):S13-S16.
Richter JA, Barankay A, Gob E, Spath P. Cardiovascular effects of ketanserin in the treatment of hypertension during coronary artery by-pass surgery. J Cardiovasc Pharmacol 1986;8:109–115.
Monopoli A, Forlani A, Bevilacqua M, et al. Interaction of selected vasodilating beta-blockers with adrenergic receptors in human cardiovascular tissues. J Cardiovasc Pharmacol 1989;14:114–120.
Vanhoutte P, Amery A, Birkenhager W, et al. Serotonin mechanism in hypertension. Focus on the effects of ketanserin. Hypertension 1988;11:111–133.
Parson AA, Whalley ET, Feniuk W, Connor HE, Humprey PPA. 5HT1-like receptors mediate 5-hydroxytryptamine-induced contractioin of human isolated basilar artery. Br J Pharmacol 1989;96:434–449.
Blauw GJ, van Brummelen P, Zwieten PA. Serotonin induced vasodilatation in the human forearm is antagonized by the selective 5-HT3 receptor antagonist. Life Sci 1988;43:1441–1449.
Conti A, Monopoli A, Forlani A, Antona C, Biglioli P. Vasoconstriction induced by 5-hydroxytryptamine in the human mammary artery is mediated by 5-HT2 receptor subtype. Eur J Pharmacol 1989, in press.
van de Starre PJA, Reneman RS. The α-adrenergic receptor blocking effect of ketanserin and the interaction between α-adrenergic and S2-serotoninergic receptor blockade. J Cardiovasc Pharmacol 1988;11 (Suppl 1): S54-S61.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Monopoli, A., Conti, A., Forlani, A. et al. 5-Hydroxytryptamine induces contraction in isolated human mammary artery: Effect of ketanserin. Cardiovasc Drug Ther 4, 59–61 (1990). https://doi.org/10.1007/BF00053428
Issue Date:
DOI: https://doi.org/10.1007/BF00053428