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Preliminary studies on the effect of Anaplasma marginale antibodies ingested by Dermacentor andersoni ticks (Acari:Ixodidae) with their blood meal on infections in salivary glands

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Abstract

The effect of Anaplasma marginale antibodies ingested with the tick blood meal was tested on infected male ticks that were allowed to feed on cattle immunized with the erythrocytic stage of A. marginale. The experiments were done in two trials. Trial 1 was done using splenectomized calves (two calves per treated and control groups) while ticks in trial 2 were fed on intact yearling cattle (four cattle per treated and control groups). The cattle were immunized with purified outer membrane proteins of erythrocyte-derived A. marginale using saponin (trial 1) or monophosphoryl lipid-A-trehalose dicorynomycolate adjuvant (trial 2). The corresponding control cattle received adjuvant only. All cattle were challenged using Dermacentor andersoni males infected as adults that were allowed to feed for 7 days. In trial 1, the ticks were allowed to feed a second time on susceptible calves to test whether exposure of ticks to immunized cattle affected their ability to transmit anaplasmosis. Infections in fed ticks were monitored by determining the infection rates in salivary glands with an A. marginale-specific RNA probe and light microscopy. Vaccine-derived antibodies ingested with the tick blood meal did not appear to affect the development of A. marginale in previously infected ticks. The infection rates in the salivary glands were not significantly different among ticks fed on immunized versus adjuvant control cattle. When the vaccine-exposed ticks in trial 1 were allowed to feed a second time on susceptible calves, the resulting clinical symptoms of anaplasmosis were similar to those of the controls. There was no statistically significant effect of tick exposure to the anti-erythrocytic stage antibody on the development of salivary gland infection or transmission of A. marginale by ticks.

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Authors and Affiliations

  1. Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, 74078, Stillwater, Oklahoma, USA

    Katherine M. Kocan, Edmour F. Blouin, Wanda L. Edwards & P. L. Claypool

  2. Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, 99164, Pullman, Washington, USA

    Guy H. Palmer & Inge S. Eriks

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  1. Katherine M. Kocan
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  2. Edmour F. Blouin
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  3. Guy H. Palmer
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  4. Inge S. Eriks
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  5. Wanda L. Edwards
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  6. P. L. Claypool
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Kocan, K.M., Blouin, E.F., Palmer, G.H. et al. Preliminary studies on the effect of Anaplasma marginale antibodies ingested by Dermacentor andersoni ticks (Acari:Ixodidae) with their blood meal on infections in salivary glands. Exp Appl Acarol 20, 297–311 (1996). https://doi.org/10.1007/BF00052960

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