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First data on resistance mechanisms of Varroa jacobsoni (OUD.) against tau-fluvalinate

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Abstract

In 1991, the first losses of efficacy of tau-fluvalinate against the honeybee ectoparasite Varroa jacobsoni Oud. were recorded in Sicily. Since then, diminished efficacy with available pyrethroid treatments has been encountered in many regions of Italy. The aim of this study was to investigate the type of resistance in V. jacobsoni to the pyrethroid tau-fluvalinate by focusing on metabolic resistance mechanisms (detoxication). After developing a suitable application method, two synergists were used: piperonyl butoxide (PBO), as an inhibitor of the microsomal monooxygenases of the cytochrome P450 complex and S,S,S-tributylphosphorotrithioate (DEF), which blocks esterases. A significant decrease in the LC50 values of the susceptible and of the resistant mite strains after the application of PBO was observed. A slight decrease of the LC50 values was also observed after the application of DEF. However, this decrease was not significant. These results indicate that the resistance of Varroa mites to tau-fluvalinate can partly be explained by an increased detoxication due to the monooxygenases in the P450 system, which is blocked by PBO. Esterases seems to play a negligible role. Whether glutathione-S-transferases are involved, is still unknown, but other mechanisms, such as the modification of the binding sites and/or reduced uptake might be involved as well.

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References

  • Bassand, D. 1993. Du bon ou mauvais usage de Fluvalinate contre Varroa jacobsoni: etude des risques d'appartion d'une résistance. J. De 1;Abeille de France, 784: 313–316.

    Google Scholar 

  • Beetsma, J. 1994. The Varroa mite, a devastating parasite of Western honeybees and an economic threat to beekeeping. Outlook Agricult., 23: 169–175.

    Google Scholar 

  • Berkson, J. 1953. A statistically precise and relatively simple method of estimating the bioassay with quantal response, based on the logistic function. J. Am. Stat. Assoc., 48: 565–599.

    Google Scholar 

  • Boecking, O. and Ritter, W. 1994. Current status of behavioral tolerance of the honey bee Apis mellifera to the mite Varroa jacobsoni. Am. Bee J., 134: 689–694.

    Google Scholar 

  • Chilcutt, C.F. and Tabashnik, B.E. 1995. Evolution of pesticide resistance and slope of the concentration-mortality line: are they related? J. Econ. Entomol., 88: 11–20.

    Google Scholar 

  • Dobzhansky, T. 1951. Genetics and the Origin of the Species, 3rd edn., Columbia University Press, New York.

    Google Scholar 

  • Engels, W. and Rosenkranz, P. 1992. Hyperthermic experiences in control of varroasis. Apidologie 23 (4): 379–381.

    Google Scholar 

  • Falconer, D. 1989. Introduction to Quantative Genetics, 3rd edn. Longman, New York.

    Google Scholar 

  • Hassall, K.A. 1990. The Biochemistry and Uses of Pesticides. VCH, New York.

    Google Scholar 

  • Hoffman, S., Büchler, R., Bienefeld, K. and Urfer, W. 1995. Genetische Effekte auf den Varroabefall innerhalb der Carnica-Population. In Kongressband zum 24. Internationalem Bienenzüchterkongress—Programm und Kurzfassung der Referate p. 97.

  • Imdorf, A., Kilchenmann, V., Bogdanov, S., Bachofen, S. and Beretta, C. 1995. Toxizität von Thymol, Campher, Menthol and Eucalyptol auf Varroa jacobsoni Oud. und Apis mellifera L. im Labortest. Apidologie 26 (1): 27–31.

    Google Scholar 

  • Lande, R. 1981. The minimum number of genes contributing to quantitative variation between and within populations. Genetics 99: 541–553.

    Google Scholar 

  • Linder, A. and Berchtold, W. 1976. Statistische Auswertung von Prozentzahlen. Probit und Logitanalyse mit EDV, 1st edn. Birkhäuser Verlag, Basel.

    Google Scholar 

  • Lodesani, M., Colombo, M. and Spreafico, M. 1995. Ineffectiveness of Apistan® treatment against the mite Varroa jacobsoni Oud. in several districts of Lombardy (Italy). Apidologie 26 (1): 67–72.

    Google Scholar 

  • Loglio, G. and Plebani, G. 1992. Valutazione dell'efficacia dell'Apistan. Apicult. Mod. 83: 95–98.

    Google Scholar 

  • Milani, N. 1995. The resistance of Varroa jacobsoni Oud to pyrethroids: a laboratory assay. Apidologie 26 (6): 415–429.

    Google Scholar 

  • Omer, A.D., Tabashnik, B.E., Johnson, M.W. and Leigh, F. 1993. Realized heritability of resistance to dicrotophos in greenhouse whitefly. Entomol. Exp. Appl. 68: 65–73.

    Google Scholar 

  • Raffa, K.F. and Priester, T.M. 1985. Synergists as research tolls and control agents in agriculture. J. Agricult. Entomol. 2: 27–45.

    Google Scholar 

  • Ritter, W. 1996. Diagnose und Therapie der Bienenkrankheiten. G. Fischer Verlag, Jena.

    Google Scholar 

  • Ritter, W. and Roth, H. 1988. Experiments with mite resistance to varroacidal substances in the laboratory. Proceedings of a Meeting of the EC Experts' Group /Bad Homburg, 15–17 October 1986. In European research on varroatosis control, R. Cavalloro, A.A. Balkema, Rotterdam/Brookfield.

    Google Scholar 

  • Ritter, W., Michel, P., Schwendenmann, A. and Bartoldi, M. 1990. Entwicklung des Befalls mit Varroa jacobsoni O. bei Bienenvölkern in Tunesien. Berl. Münch. Tierärztl. Wschur. 103: 109–111.

    Google Scholar 

  • Robertson, J.L. and Preisler, H.K. 1992. Pesticide Bioassays with Arthropods. CRC Press, London.

    Google Scholar 

  • Rosenheim, J.A. and Hoy, M.A. 1986. Intraspecific variation in levels of pesticide resistance in field populations of a parasitoid, Aphytis melinus (Hymenoptera: Aphelinidae): the role of past selection pressures. J. Econ. Entomol. 79: 1161–1173.

    Google Scholar 

  • Sachs, L. 1984. Angewandte Statistik, 6th ed. Springer-Verlag, Berlin.

    Google Scholar 

  • Scott, J.G. 1990. Investigating mechanisms of insecticide resistance: method, strategies and pitfalls. In Pesticide resistance in arthropods, R.T., Roush and B.E., Tabashnik (eds), pp. 39–57. Chapman & Hall, New York.

    Google Scholar 

  • Soderlund, D.M. and Bloomquist, J.R. 1990. Molecular mechanisms of insecticide resistance. In Pesticide resistance in arthropods, R.T., Roush and B.E., Tabashnik (eds), pp. 58–96. Chapman & Hall, New York.

    Google Scholar 

  • Tabashnik, B.E. and McGaughey, W.H. 1994. Resistance risk assessment for single and multiple insecticides: responses of Indian meal moth (Lepidoptera: Pyralidae) to Bacilus thurigiensis. J. Econ. Entomol. 86: 635–644.

    Google Scholar 

  • Trouiller, J. 1995. Monitorisierung der Apistan-Wirksamkeit im Süden Europas. In Kongressband zum 24. Internationalem Bienenzüchterkongress—Programm und Kurzfassung der Referate, p. 109.

  • Vandame, R., Colin, M.E., Belzunces, L.P. and Jourdan, P. 1995. Résistance de Varroa au fluvalinate. Le Carnet Européen, 3: 5–11.

    Google Scholar 

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Hillesheim, E., Ritter, W. & Bassand, D. First data on resistance mechanisms of Varroa jacobsoni (OUD.) against tau-fluvalinate. Exp Appl Acarol 20, 283–296 (1996). https://doi.org/10.1007/BF00052878

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