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Mitochondrial DNA RFLP in genus Oryza and cultivated rice

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Summary

Ninety-three accessions representing 23 species from the genus Oryza were surveyed for restriction fragment length polymorphism (RFLP) in mitochondrial (mt) DNA by probing total DNA with 15 known mt sequences cloned in plasmids from higher plants, and five mt genomic cosmid clones from maize. Very low levels of intra-specific and even intra-cytologically-defined nuclear genome mt DNA RFLP were found. High between-genome differentiation appeared, suggesting phylogenetic relationships consistent with data from previous nuclear and chloroplast (cp) DNA studies. Parallel inheritance of cp and mt DNA was found. There was one major exception: the mt DNA of the allotetraploid CD genome is apparently equally related to two putative diploid progenitors, which is suggestive of an interspecific recombination.

RRLP in mt DNA was also probed in 82 cultivars, with four plasmid probes. Some bands not seen in the wild species appeared in O. sativa, with intra-specific polymorphism relatively higher than in the wild species. The pattern of variation paralleled that at the cp DNA level between the indica and japonica subspecies.

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  1. G. Second

    Present address: ORSTOM, Laboratoire Ressources Généiques et Amélioration des Plantes Tropicales, BP5045, F34032, Montpellier, France

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  1. Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, 14853, Ithaca, NY, USA

    G. Second & Z. Y. Wang

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Second, G., Wang, Z.Y. Mitochondrial DNA RFLP in genus Oryza and cultivated rice. Genet Resour Crop Evol 39, 125–140 (1992). https://doi.org/10.1007/BF00051926

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